Anti-AKT1 Mouse Monoclonal Antibody [Clone ID: OTI4D6]

Figure 1. Western blot analysis of AKT1 using anti-AKT1 antibody (M00024).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human A549 whole cell lysates,
Lane 2: human MCF-7 whole cell lysates,
Lane 3: human HepG2 whole cell lysates,
Lane 4: human Raji whole cell lysates,
Lane 5: rat brain tissue lysates,
Lane 6: rat PC-12 whole cell lysates,
Lane 7: mouse brain tissue lysates,
Lane 8: mouse NIH/3T3 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-AKT1 antigen affinity purified monoclonal antibody (Catalog # M00024) at at 1:51000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for AKT1 at approximately 56 kDa. The expected band size for AKT1 is at 56 kDa.

Equivalent amounts of cell lysates (10 ug per lane) of wild-type Hela cells (WT) and AKT1-Knockout Hela cells (KO) were separated by SDS-PAGE and immunoblotted with anti-AKT1 monoclonal antibody M00024

Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-AKT1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer

Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-AKT1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer

Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-AKT1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer

Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-AKT1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-AKT1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer

Anti-AKT1 mouse monoclonal antibody (M00024) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY AKT1.

Flow cytometric Analysis of Jurkat cells