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- Table of Contents
AKT1 antibody (M00024-1) performs well in Flow Cytometry, IP, IF, IHC, ICC, WB and shows reactivity with Human, Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 25 Publications
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Immunofluorescent analysis of Hela cells, using AKT1 Antibody.
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Anti-AKT1 Monoclonal Antibody
AKT1 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse, Rat | Rabbit | Flow Cytometry, IP, IF, IHC, ICC, WB |
Tested Applications
AKT1 antibody was positively detected by WB in:
human HepG2 whole cell, human A549 whole cell, human Hela whole cell, human Raji whole cell, human MCF-7 whole cell, human SiHa whole cell, human 293T whole cell, rat liver tissue, rat brain tissue, rat C6 whole cell, rat RH35 whole cell, mouse liver tissue, mouse brain tissue, Mouse Neuro-2a whole cell, Mouse HEPA1-6 whole cell
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
AKT1 antibody was positively detected by IHC in:
human colon tissue
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
Recommend Dilution
Application
Dilution
Species
WB 1:500-2000
IHC 1:50-200
ICC/IF 1:50-200
IP 1:50
FC 1:50
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Reactive Data
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WB
IHC
ICC
IF
IP
FC
Human
Tested
Tested
Recommend
Recommend
Recommend
Recommend
Rat
Tested
Recommend
Recommend
Recommend
Recommend
Mouse
Tested
Recommend
Recommend
Recommend
Recommend
Product Information
Product Name
Anti-AKT1 Monoclonal Antibody
View all AKT1 Antibodies
View all AKT1 Antibodies
Form
Liquid
Uniprot
Gene ID
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
EBG-1
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human AKT1 Akt, also referred to as PKB or Rac, plays a critical role in controlling survival and apoptosis. This protein kinase is activated by insulin and various growth and survival factors to function in a wortmannin-sensitive pathway involving PI3 kinase. Akt is activated by phospholipid binding and activation loop phosphorylation at Thr308 by PDK1 and by phosphorylation within the carboxy terminus at Ser473.
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
56 kDa
Calculated Molecular Weight
55.7 kDa
Cite This Product
Anti-AKT1 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00024-1)
Protocols
Western Blot protocol for AKT1 antibody
Immunohistochemistry (IHC) protocol for AKT1 antibody
Publications
Application:
Species:
Study on the percutaneous absorption and synergistic anti-rheumatoid arthritis effect of the Zanthoxylum bungeanum essential oil on Qin Jiao-Du Huo
Published: 2026-05-25
Journal: FITOTERAPIA
Application: WB
Impact Factor: 2.90
Species: Human
PMID: 42184869
YTHDF3 Mediates the Occurrence and Development of Breast Cancer by Regulating Glycolysis Through the mTOR–HIF1α–LHDA Axis
Published: 2026-04-26
Journal: JOURNAL OF CELLULAR AND MOLECULAR MEDICINE
Application: WB
Impact Factor: 4.20
Species: Human
PMID: 42036761
The Combination of Phytic Acid and Inositol Alleviates Metastasis of Colorectal Cancer in Mice by Inhibiting PI3K/AKT Pathway and M2 Macrophage Polarization
Published: 2025-12-02
Journal: BIOLOGICAL & PHARMACEUTICAL BULLETIN
Application: WB
Impact Factor: 1.70
Species: Mouse
PMID: 41339008
Mulberry leaf flavonoid extract inhibits high glucose-induced ferroptosis by regulating the PI-3K/Akt/Nrf2/GPX4 pathway in HT22 mouse hippocampal neuronal cells
Published: 2025-11-29
Journal: EXPERIMENTAL CELL RESEARCH
Application: WB
Impact Factor: 3.50
Species: Mouse
PMID: 41325790
Luteolin alleviates right ventricular hypertrophy in high altitude pulmonary hypertension rats by regulating PI3K/AKT/mTOR signalling pathway
Published: 2025-07-01
Journal: EUROPEAN JOURNAL OF PHARMACOLOGY
Application: WB
Impact Factor: 4.70
Species: Rat
PMID: 40609617
Bioactive compound combinations from Rhodiola tangutica alleviate pulmonary vascular remodeling in high-altitude pulmonary hypertension rats through the PI3K–AKT pathway
Published: 2025-05-02
Journal: Frontiers in Pharmacology
Application: WB
Impact Factor: 4.80
Species: Rat
PMID: 40385484
Aloperine treatment attenuates acute spinal cord injury by reducing oxidative, inflammatory, and apoptotic responses via PI3K/AKT/NF-κB signaling in a rat contusion model
Published: 2025-03-23
Journal: NEUROSCIENCE LETTERS
Application: WB
Impact Factor: 2.00
Species: Rat
PMID: 40132779
Rosmarinic acid attenuates glioblastoma cells and spheroids’ growth and EMT/stem-like state by PTEN/PI3K/AKT downregulation and ERK-induced apoptosis.
Published: 2024-09-19
Journal: PHYTOMEDICINE
Application: WB
Impact Factor: 6.70
Species: Human
PMID: 39341126
Neuregulin 4 (Nrg4) cooperates with melatonin to regulate the PRL expression via ErbB4/Erk signaling pathway as a potential prolactin (PRL) regulator
Published: 2024-03-11
Journal: JOURNAL OF CELLULAR BIOCHEMISTRY
Application: WB
Impact Factor: 3.00
Species: Rat
PMID: 38465779
A breakthrough in periodontitis treatment: Revealing the pharmacodynamic substances and mechanisms of Kouqiangjie formula
Published: 2024-01-09
Journal: JOURNAL OF ETHNOPHARMACOLOGY
Application: WB
Impact Factor: 4.80
Species: Rat
PMID: 38199336
PI3K-CCL2-CCR2-MDSCs axis: A potential pathway for tumor Clostridia-promoted CD 8+ T lymphocyte infiltration in bile tract cancers
Published: 2023-07-27
Journal: NEOPLASIA
Application: IHC,WB
Impact Factor: 4.80
Species: Human
PMID: 37515847
A prognostic biomarker NRG1 promotes U‑87 MG glioblastoma cell malignancy by inhibiting autophagy via ERBB2/AKT/mTOR pathway
Published: 2023-07-14
Journal: JOURNAL OF CELLULAR BIOCHEMISTRY
Application: WB
Impact Factor: 4.00
Species: Human
PMID: 37450666
Sulfathiazole treats type 2 diabetes by restoring metabolism through activating CYP19A1
Published: 2023-01-07
Journal: BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS
Application: WB
Impact Factor: 3.00
Species: Human
PMID: 36627088
Hsp27, Hsp60, Hsp70, or Hsp90 depletion enhances the antitumor effects of resveratrol via oxidative and ER stress response in human glioblastoma cells
Published: 2023-01-02
Journal: BIOCHEMICAL PHARMACOLOGY
Application: WB
Impact Factor: 5.80
Species: Human
PMID: 36603687
Resveratrol inhibits paclitaxel-induced neuropathic pain by the activation of PI3K/Akt and SIRT1/PGC1α pathway
Published: 2022-12-23
Journal: Journal of Pain Research
Application: IHC
Impact Factor: 2.83
Species: Rat
PMID: 30881098
Effect of Diammonium Glycyrrhizinate in Improving Focal Cerebral Ischemia-Reperfusion Injury in Rats Through Multiple Mechanisms
Published: 2022-12-01
Journal: Dose-Response
Application: IHC
Impact Factor: 2.62
Species: Rat
PMID: 36479318
G protein-coupled receptor 30 mediates cell proliferation of goat mammary epithelial cells via MEK/ERK&PI3K/AKT signaling pathway
Published: 2022-06-06
Journal: CELL CYCLE
Application: WB
Impact Factor: 5.17
Species: Goat
PMID: 35659445
Effects of 4-vinylcyclohexene diepoxide on the cell cycle, apoptosis, and steroid hormone secretion of goat ovarian granulosa cells
Published: 2022-04-06
Journal: IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL
Application: WB
Impact Factor: 2.72
Species: Goat
PMID: 35386089
Cichorium pumilum Jacq Extract Inhibits LPS-Induced Inflammation via MAPK Signaling Pathway and Protects Rats From Hepatic Fibrosis Caused by Abnormalities in the Gut-Liver Axis
Published: 2021-04-29
Journal: Frontiers in Pharmacology
Application: WB
Impact Factor: 5.81
Species: Mouse
PMID: 33995112
The calcimimetic R-568 attenuates subarachnoid hemorrhage-induced vasospasm through PI3K/Akt/eNOS signaling pathway in the rat model
Published: 2021-04-28
Journal: BRAIN RESEARCH
Application: WB
Impact Factor: 3.25
Species: Rat
PMID: 33930376
Hydrogen sulfide ameliorates doxorubicin‑induced myocardial fibrosis in rats via the PI3K/AKT/mTOR pathway
Published: 2021-02-24
Journal: Molecular Medicine Reports
Application: WB
Impact Factor: 2.95
Species: Rat
PMID: 33649809
Mechanism of Action of Xiaoyao San in Treatment of Ischemic Stroke is Related to Anti-Apoptosis and Activation of PI3K/Akt Pathway
Published: 2021-02-22
Journal: Drug Design Development and Therapy
Application: WB
Impact Factor: 4.16
Species: Human
PMID: 33654381
Apoptin-derived peptide reverses cisplatin resistance in gastric cancer through the PI3K–AKT signaling pathway
Published: 2018-03-09
Journal: Cancer Medicine
Application: WB,IHC
Impact Factor: 3.20
Species: Human
PMID: 29522284
Metabolic syndrome in rats is associated with erectile dysfunction by impairing PI3K/Akt/eNOS activity
Published: 2017-10-18
Journal: Scientific Reports
Application: WB,IHC,IF
Impact Factor: 4.26
Species: Rat
PMID: 29044143
Expression of TGF-β1/mTOR signaling pathway in pathological scar fibroblasts
Published: 2017-04-05
Journal: Molecular Medicine Reports
Application: IF
Impact Factor: 1.69
Species: Human
PMID: 28393182
Review
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3 Reviews For Anti-AKT1 Monoclonal Antibody
The AKT1 Antibody (M00024-1) showed clear, specific bands in WB of rat skeletal muscle, with expression changes consistent with expectations.
| SKU | M00024-1 |
|---|---|
| Application | Western Blot |
| Sample | Rat skeletal muscle tissue |
| Sample Processing Description | Normal rat skeletal muscle, injury model, and high-, medium-, and low-dose treatment groups, with total protein extracted. |
| Other Reagents | RIPA lysis buffer, Protease inhibitor, Running buffer, Transfer buffer, Blocking buffer |
| Primary Antibody | AKT1 Monoclonal Antibody |
| Primary Incubation | 1:2000, overnight at 4 ℃ |
| Secondary Antibody | HRP Conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) (BA1054) |
| Secondary Incubation | 1:10000, 1 h in RT |
| Detection | Substrate: ECL substrate, Image system: ChemiDoc MP |
| Results Summary | The AKT1 bands are clear and correctly positioned; expression is significantly higher in the model group than in the control, and decreases with increasing drug dose in the treatment groups, consistent with expectations. |
Guangtian Yu, Ningxia Medical University
Verified customer
Submitted 2026-03-25
This antibody is highly specific and efficient, suitable for detecting AKT1 protein in rat colon by Western blot, with only minor nonspecific bands.
| SKU | M00024-1 |
|---|---|
| Application | Western Blot |
| Sample | rat colon tissue |
| Sample Processing Description | RIPA lysis buffer: Add protease inhibitor PMSF (100:1), lyse for 10 min, centrifuge at 12,000 rpm for 15 min, transfer the supernatant and mix with 5× loading buffer, denature at 100 °C for 10 min, and load onto SDS-PAGE. |
| Other Reagents | Blocking buffer |
| Primary Antibody | AKT1 Monoclonal Antibody |
| Primary Incubation | 1:1000, overnight at 4 ℃ |
| Secondary Antibody | HRP Conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) |
| Secondary Incubation | 1:1000, 1 hour in room temperature |
| Detection | Substrate: ECL, Imaging system:ChemiDoc MP |
| Results Summary | The figure shows a schematic of Western blot results for the target protein AKT1 and the internal control Actin in rat colon across different groups. No significant differences were observed between groups; the target bands are clear and distinct, and the experimental results are satisfactory. |
Shiyu Zhang, LUTCM
Verified customer
Submitted 2025-12-29
Western blot analysis of AKT1 protein in mouse hippocampus using the AKT1 antibody showed clear bands. The antibody is suitable for mouse hippocampal tissue samples.
| SKU | M00024-1 |
|---|---|
| Application | Western Blot |
| Sample | Mouse hippocampus tissue |
| Sample Processing Description | The mouse hippocampus was lysed with RIPA buffer containing a protease inhibitor cocktail. After protein quantification, samples were mixed with 5× protein loading buffer and heated for 10 minutes to denature. Load 5 μL of protein per lane and apply to SDS-PAGE. |
| Primary Antibody | Anti-AKT1 Monoclonal Antibody |
| Primary Incubation | overnight at 4 ℃ |
| Secondary Antibody | HRP-conjugated Anti-Rabbit IgG Secondary Antibody |
| Secondary Incubation | 1 hour in room temperature |
| Detection | Substrate: Ultra-sensitive ECL luminescent reagent (Cat# AR1191), Imaging system: ChemiDoc MP (Bio-Rad) |
| Results Summary | Western blot analysis of AKT1 protein in the mouse hippocampus using the AKT1 antibody showed clear bands. This antibody is suitable for mouse hippocampal tissue samples. |
Changbin Yuan, LNUTCM
Verified customer
Submitted 2025-11-05
Product Promise
We promise Anti-AKT1 Monoclonal Antibody performs excellently in relevant applications for Flow Cytometry, IP, IF, IHC, ICC, WB. If it doesn't perform as promised, we will refund the product. Learn more
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M00024-1
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
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Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 1, 2026