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Product Info Summary
| SKU: | M08868 |
|---|---|
| Size: | 80 µl |
| Reactive Species: | Human |
| Host: | Mouse |
| Application: | IF, IHC-P, WB |
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Product info
Product Name
Anti-ALDH6A1 Antibody
SKU/Catalog Number
M08868
Size
80 µl
Description
ALDH6A1 encodes mitochondrial methylmalonate semialdehyde dehydrogenase, participating in valine and pyrimidine catabolic pathways by converting malonate/methylmalonate semialdehydes toward acetyl-/propionyl-CoA. Assay context: human-reactive antibody tested for WB, IF, and paraffin IHC, supporting compartmental mapping of mitochondrial metabolism in tissue sections and lysates. Often grouped with aldehyde-stress handling enzymes such as ALDH3A2 and ALDH7A1 to separate pathway-specific aldehyde flux from broader detox programs (putative).
Storage & Handling
Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Cite This Product
Anti-ALDH6A1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M08868)
Host
Mouse
Contents
Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clonality
Monoclonal
Clone Number
147CT8.3.4
Isotype
IgG1,IgK
Immunogen
This ALDH6A1 antibody is generated from mouse immunized with ALDH6A1 recombinant protein.
Reactive Species
M08868 is reactive to ALDH6A1 in Human
Calculated molecular weight
57.8 kDa
Background of ALDH6A1
This protein belongs to the aldehyde dehydrogenases family of proteins. This enzyme plays a role in the valine and pyrimidine catabolic pathways. The product of this gene, a mitochondrial methylmalonate semialdehyde dehydrogenase, catalyzes the irreversible oxidative decarboxylation of malonate and methylmalonate semialdehydes to acetyl- and propionyl-CoA. Methylmalonate semialdehyde dehydrogenase deficiency is characterized by elevated beta-alanine, 3-hydroxypropionic acid, and both isomers of 3-amino and 3-hydroxyisobutyric acids in urine organic acids.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M08868 is guaranteed for IF, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
IF: 1:25
WB: 1:1000
IHC-P: 1:25
Validation Images & Assay Conditions
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Fluorescent image of MCF-7 cells stained with ALDH6A1 Antibody. M08868 was diluted at 1:25 dilution. An Alexa Fluor® 488-conjugated goat anti-mouse lgG at 1:400 dilution was used as the secondary antibody (green). DAPI was used to stain the cell nuclear (blue). Cytoplasmic actin was counterstained with Alexa Fluor® 555 conjugated with Phalloidin (red).
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Western blot analysis of lysates from MCF-7, T47D cell line (from left to right), using ALDH6A1 Antibody. M08868 was diluted at 1:1000 at each lane. A goat anti-mouse IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 20μg per lane.
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Immunohistochemical analysis of paraffin-embedded H.colon section using ALDH6A1 Antibody. M08868 was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-mouse IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
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Immunohistochemical analysis of paraffin-embedded H.liver section using ALDH6A1 Antibody. M08868 was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-mouse IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
Specific Publications For Anti-ALDH6A1 Antibody (M08868)
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