Anti-Alpha Actinin 4/ACTN4 Antibody Picoband™

Boster Bio Anti-Alpha Actinin 4/ACTN4 Antibody Picoband™ catalog # PB9974. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat.

Product Info Summary

SKU: PB9974
Size: 100μg/vial
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: IHC, WB

Product Name

Anti-Alpha Actinin 4/ACTN4 Antibody Picoband™

See all Alpha Actinin 4 products

SKU/Catalog Number

PB9974

Size

100μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Alpha Actinin 4/ACTN4 Antibody Picoband™ catalog # PB9974. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Alpha Actinin 4/ACTN4 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9974)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Immunogen

E.coli-derived human Alpha Actinin 4 recombinant protein (Position: E561-V661). Human Alpha Actinin 4 shares 99% and 98% amino acid (aa) sequence identity with mouse and rat Alpha Actinin 4, respectively.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross Reactivity

No cross reactivity with other proteins

Reactive Species

PB9974 is reactive to ACTN4 in Human, Mouse, Rat

Applications

PB9974 is guaranteed for IHC, WB Boster Guarantee

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat, By Heat
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat

Validation Images & Assay Conditions

Gene/Protein Information For ACTN4 (Source: Uniprot.Org, NCBI)

Uniprot ID

O43707

Gene ID

81

Gene Name

ACTN4

Full Name

Alpha-actinin-4

Weight

104.854kDa

Superfamily

alpha-actinin family

Alternative Names

actinin alpha4 isoform; actinin, alpha 4; ACTININ-4; alpha-actinin-4; DKFZp686K23158; F-actin cross-linking protein; focal segmental glomerulosclerosis 1; FSGS; FSGS1; Non-muscle alpha-actinin 4 ACTN4 ACTININ-4, FSGS, FSGS1 actinin alpha 4 alpha-actinin-4|focal segmental glomerulosclerosis 1|non-muscle alpha-actinin 4

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on ACTN4, check out the ACTN4 Infographic

ACTN4 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for ACTN4: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

No publications found for PB9974

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7 Customer Q&As for Anti-Alpha Actinin 4/ACTN4 Antibody Picoband™

Question

Do you have a BSA free version of anti-Alpha Actinin 4/ACTN4 antibody PB9974 available?

Verified Customer

Verified customer

Asked: 2020-04-02

Answer

Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-Alpha Actinin 4/ACTN4 antibody PB9974 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2020-04-02

Question

Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for neuroblastoma using anti-Alpha Actinin 4/ACTN4 antibody PB9974. Let me know if you need anything else.

Verified Customer

Verified customer

Asked: 2019-09-18

Answer

We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-09-18

Question

My question regarding product PB9974, anti-Alpha Actinin 4/ACTN4 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2019-09-11

Answer

We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PB9974 anti-Alpha Actinin 4/ACTN4 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2019-09-11

Question

I have attached the WB image, lot number and protocol we used for neuroblastoma using anti-Alpha Actinin 4/ACTN4 antibody PB9974. Please let me know if you require anything else.

Verified Customer

Verified customer

Asked: 2019-07-10

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-07-10

Question

We are currently using anti-Alpha Actinin 4/ACTN4 antibody PB9974 for rat tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on monkey tissues as well?

Verified Customer

Verified customer

Asked: 2019-05-28

Answer

The anti-Alpha Actinin 4/ACTN4 antibody (PB9974) has not been tested for cross reactivity specifically with monkey tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2019-05-28

Question

Is this PB9974 anti-Alpha Actinin 4/ACTN4 antibody reactive to the isotypes of ACTN4?

Verified Customer

Verified customer

Asked: 2018-05-16

Answer

The immunogen of PB9974 anti-Alpha Actinin 4/ACTN4 antibody is E.coli-derived human Alpha Actinin 4 recombinant protein (Position: E561-V661). Human Alpha Actinin 4 shares 99% and 98% amino acid (aa) sequence identity with mouse and rat Alpha Actinin 4, respectively. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2018-05-16

Question

Would PB9974 anti-Alpha Actinin 4/ACTN4 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

C. Jones

Verified customer

Asked: 2015-08-03

Answer

It shows on the product datasheet, PB9974 anti-Alpha Actinin 4/ACTN4 antibody as been tested on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2015-08-03

Conjugation

Size

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Ships directly to USA and Canada. For other regions, visit our distributors page

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Sample Inquiry

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PB9974
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$280.00

$50 fee for conjugation. Antibody size is reduced to 50ug.

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