Anti-αB-Crystallin Antibody

Product Name

Anti-αB-Crystallin Antibody

View all AlphaB Crystallin/CRYAB Antibodies

SKU/Catalog Number

A03496-1

Size

50µg

Form

Liquid. In PBS, pH 7.2, containing 50% glycerol and 0.09% sodium azide.

Description

Rabbit Polyclonal antibody for CRYAB detection. Tested positive for IF, IHC, WB in Human, Mouse, Rat

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-αB-Crystallin Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03496-1)

Host

Rabbit

Contents

Liquid. In PBS, pH 7.2, containing 50% glycerol and 0.09% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Immunogen

Synthetic peptide corresponding to the sequence near the C-terminus of human αB-Crystallin. The sequence is completely conserved in rat, rabbit, and bovine.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A03496-1 is reactive to CRYAB in Human, Mouse, Rat

Applications

A03496-1 is guaranteed for IF, IHC, WB Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

20.159kDa

Background of AlphaB Crystallin/CRYAB

Anti-Glycogen Synthase 1 pS641 antibody is validated by IHC, Western Blot and ELISA. Human muscle glycogen synthase (GS) is responsible for the biosynthesis of glycogen from phosphorylated glucose units. Mammalian liver and muscle contain GS consisting of four subunits with a total molecular weight of 360,000. GS is subject to regulation through both allosteric and covalent modification and occurs in two forms: the phosphorylated inactive form, and the dephosphorylated active form. GS is inactivated by the serine/threonine kinase called glycogen synthase kinase-3 that mainly functions to phosphorylate muscle glycogen synthase. This antibody is specific for the phosphorylated form of GS at S641. Phosphorylation of GS at S641 has been associated with Antiphospholipid Antibody Syndrome.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western Blot (1:1,000, colorimetric)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.

Validation Images & Assay Conditions

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Figure 1. IHC analysis of CRYAB using anti-CRYAB antibody (A03496-1).
CRYAB was detected in paraffin-embedded section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-CRYAB Antibody (A03496-1) overnight at 4°C. Biotinylated goat anti Rabbit IgG antibody was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

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Figure 2. Western blot analysis of CRYAB using anti-CRYAB antibody (A03496-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CRYAB antigen affinity purified polyclonal antibody (Catalog # A03496-1) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-Rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # SA1022) with Tanon 5200 system. A specific band was detected for CRYAB.

Gene/Protein Information For CRYAB (Source: Uniprot.org, NCBI)

Alternative Names

alpha B crystallin; alpha(B)-crystallin; AlphaB Crystallin; alpha-crystallin B chain; CRYA2; CRYA2alpha crystallin B chain; CRYAB; crystallin, alpha B; CTPP2; Heat shock protein beta-5; heat-shock 20 kD like-protein; HSPB5; Renal carcinoma antigen NY-REN-27; Rosenthal fiber component CRYAB CMD1II, CRYA2, CTPP2, CTRCT16, HEL-S-101, HSPB5, MFM2 crystallin alpha B alpha-crystallin B chain|epididymis secretory protein Li 101|heat shock protein beta-5|heat-shock 20 kD like-protein|renal carcinoma antigen NY-REN-27|rosenthal fiber component

For more info on CRYAB, check out the CRYAB Infographic

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In this infographic, you will see the following information for CRYAB: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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