Click image to see more details
Product Info Summary
| SKU: | A06852 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, ICC, WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-AMOTL2 Antibody Picoband®
SKU/Catalog Number
A06852
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-AMOTL2 Antibody Picoband® catalog # A06852. Tested in ELISA, IF, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-AMOTL2 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A06852)
Host
Rabbit
Contents
Each vial contains antibody formulated with stabilizing components, 0.9mg NaCl, 0.2mg Na2HPO4, 0.01mg NaN3.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E. coli-derived human AMOTL2 recombinant protein (Position: A401-Q480).
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A06852 is reactive to AMOTL2 in Human, Mouse, Rat
Observed Molecular Weight
130-150 kDa
Calculated molecular weight
85.8 kDa
Background of AMOTL2
Angiomotin-like protein 2 is a protein that in humans is encoded by the AMOTL2 gene. Angiomotin is a protein that binds angiostatin, a circulating inhibitor of the formation of new blood vessels (angiogenesis). It mediates angiostatin inhibition of endothelial cell migration and tube formation in vitro. The protein encoded by this gene is related to angiomotin and is a member of the motin protein family. Alternative splicing results in multiple transcript variants of this gene.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A06852 is guaranteed for ELISA, IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human
Immunocytochemistry/Immunofluorescence, 5μg/ml, Human
ELISA, 0.1-0.5μg/ml, -
Positive Control
WB: human 293T whole cell, human HepG2 whole cell
ICC/IF: Hela cell
Validation Images & Assay Conditions
Click image to see more details
Validation of drug targets of proteomics and network pharmacology with western blot. A Western blot images of PIKfyve, AFF4, Amotl2, and GLP1R in the lung tissue. B – E Data were expressed as the expression ratio of PIKfyve/β-actin, AFF4/β-actin, Amotl2/β-actin, and GLP1R/β-actin (n = 3 per group). F Western blot images of HSP90AA1, MMP1, TGF-β1, JUN, P53, p-Akt, Akt, p-STAT3 and STAT3 in the lung tissue. G – M : Data were expressed as the expression ratio of HSP90AA1/β-actin, MMP1/β-actin, TGF-β1/β-actin, JUN/β-actin, P53/β-actin, p-Akt/Akt, and p-STAT3/STAT3 (n = 3 per group). * P < 0.05, ** P < 0.01 compared with CC group; # P < 0.05, ## P < 0.01 compared with PF group. C C: control group, PF bleomycin-induced PF group, GH GHSPT treated bleomycin-induced PF group, GHSPT ginseng honeysuckle superfine powdered tea, PF pulmonary fibrosis
Index in PubMed under a CC BY license. PMID: 37221600
Click image to see more details
Western blot analysis of AMOTL2 using anti-AMOTL2 antibody (A06852).
Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human 293T whole cell lysates,
Lane 2: human HepG2 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-AMOTL2 antigen affinity purified polyclonal antibody (A06852) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody (Catalog # BA1054) at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for AMOTL2 at approximately 130-150 kDa. The expected band size for AMOTL2 is at 86 kDa.
Click image to see more details
IF analysis of AMOTL2 using anti-AMOTL2 antibody (A06852).
AMOTL2 was detected in an immunocytochemical section of Hela cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-AMOTL2 Antibody (A06852) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
Specific Publications For Anti-AMOTL2 Antibody Picoband® (A06852)
Loading publications
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-AMOTL2 Antibody Picoband®?
Share your experimental results or join a short interview to earn up to $1,000 in product credits or other rewards.
0 Reviews For Anti-AMOTL2 Antibody Picoband®
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
4 Customer Q&As for Anti-AMOTL2 Antibody Picoband®
Question
Is a blocking peptide available for product anti-AMOTL2 antibody (A06852)?
Verified Customer
Verified customer
Asked: 2020-05-06
Answer
We do provide the blocking peptide for product anti-AMOTL2 antibody (A06852). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2020-05-06
Question
Will anti-AMOTL2 antibody A06852 work for WB with placenta teratocarcinoma?
Verified Customer
Verified customer
Asked: 2020-03-18
Answer
According to the expression profile of placenta teratocarcinoma, AMOTL2 is highly expressed in placenta teratocarcinoma. So, it is likely that anti-AMOTL2 antibody A06852 will work for WB with placenta teratocarcinoma.
Boster Scientific Support
Answered: 2020-03-18
Question
We are currently using anti-AMOTL2 antibody A06852 for mouse tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on dog tissues as well?
L. Li
Verified customer
Asked: 2019-07-19
Answer
The anti-AMOTL2 antibody (A06852) has not been validated for cross reactivity specifically with dog tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-07-19
Question
Does A06852 anti-AMOTL2 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2017-08-24
Answer
You can see on the product datasheet, A06852 anti-AMOTL2 antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2017-08-24
