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Product Info Summary
| SKU: | M02364 |
|---|---|
| Size: | 80 µl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IHC-P, WB |
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Product info
Product Name
Anti-AMPH Antibody (Center)
View all Amphiphysin/AMPH Antibodies
SKU/Catalog Number
M02364
Size
80 µl
Description
Boster Bio Anti-AMPH Antibody (Center) (Catalog # M02364). Tested in WB, IHC-P application(s). This antibody reacts with Human.
Storage & Handling
Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Cite This Product
Anti-AMPH Antibody (Center) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M02364)
Host
Rabbit
Contents
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
This AMPH antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 336-370 amino acids from the Central region of human AMPH.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M02364 is reactive to AMPH in Human
Reconstitution
Calculated molecular weight
76257 Da
Background of Amphiphysin/AMPH
May participate in mechanisms of regulated exocytosis in synapses and certain endocrine cell types. May control the properties of the membrane associated cytoskeleton.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M02364 is guaranteed for IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:1000
IHC-P: 1:25
Validation Images & Assay Conditions
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Anti-AMPH Antibody (Center) at 1:1000 dilution + human brain lysates
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution
Predicted band size : 76 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
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M02364 staining AMPH in Human brain tissue sections by Immunohistochemistry (IHC-P -paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.
Protein Target Info & Infographic
Gene/Protein Information For AMPH (Source: Uniprot.org, NCBI)
Gene Name
AMPH
Full Name
Amphiphysin
Weight
76257 Da
Alternative Names
Amphiphysin, AMPH, AMPH1 AMPH AMPH1 amphiphysin amphiphysin|amphiphysin (Stiff-Mann syndrome with breast cancer 128kD auto)|amphiphysin I
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on AMPH, check out the AMPH Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for AMPH: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-AMPH Antibody (Center) (M02364)
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