Anti-Angiopoietin-1/ANGPT1 Antibody Picoband™

Product Name

Anti-Angiopoietin-1/ANGPT1 Antibody Picoband™

View all Angiopoietin-1 Antibodies

SKU/Catalog Number

A00853

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Angiopoietin-1/ANGPT1 Antibody Picoband™ catalog # A00853. Tested in ELISA, WB applications. This antibody reacts with Human.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Angiopoietin-1/ANGPT1 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00853)

Host

Rabbit

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na₂HPO₄, 0.05mg NaN₃.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence in the middle region of human Angiopoietin-1, different from the related mouse sequence by three amino acids, and from the related rat sequence by two amino acids.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A00853 is reactive to ANGPT1 in Human

Applications

A00853 is guaranteed for ELISA, WB Boster Guarantee

Observed Molecular Weight

70 kDa

Calculated molecular weight

57.513kDa

Background of Angiopoietin-1

Angiopoietin 1 is a type of angiopoietin and is encoded by the gene ANGPT1. Angiopoietins are proteins with important roles in vascular development and angiogenesis. All angiopoietins bind with similar affinity to an endothelial cell-specific tyrosine-protein kinase receptor. Angiopoietin 1 is mapped to 8q23.1. The protein encoded by this gene is a secreted glycoprotein that activates the receptor by inducing its tyrosine phosphorylation. It plays a critical role in mediating reciprocal interactions between the endothelium and surrounding matrix and mesenchyme. The protein also contributes to blood vessel maturation and stability, and may be involved in early development of the heart. Angiopoietin-1 seems to play a crucial role in mediating reciprocal interactions between the endothelium and surrounding matrix and mesenchyme.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

ELISA , 0.1-0.5μg/ml, Human, -
Western blot, 0.1-0.5μg/ml, Human

Validation Images & Assay Conditions

Click image to see more details

Figure 1. Western blot analysis of Angiopoietin-1 using anti-Angiopoietin-1 antibody (A00853).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours.
lane 1: recombinant human ANGPT1 protein 1ng
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Angiopoietin-1 antigen affinity purified polyclonal antibody (Catalog # A00853) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Angiopoietin-1 at approximately 70KD. The expected band size for Angiopoietin-1 is at 57KD.

Gene/Protein Information For ANGPT1 (Source: Uniprot.org, NCBI)

Alternative Names

AGP1; AGPT; Ang1; ANG-1; angiopoietin 1; Angiopoietin-1; ANGPT1; KIAA0003 ANGPT1 AGP1, AGPT, ANG1 angiopoietin 1 angiopoietin-1|ANG-1

For more info on ANGPT1, check out the ANGPT1 Infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for ANGPT1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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