Product Info Summary
SKU: | M02103 |
---|---|
Size: | 100 μl |
Reactive Species: | Human |
Host: | Rabbit |
Application: | IP, IF, ICC, WB |
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Product info
Product Name
Anti-Angiotensinogen AGT Rabbit Monoclonal Antibody
SKU/Catalog Number
M02103
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-Angiotensinogen AGT Rabbit Monoclonal Antibody catalog # M02103. Tested in WB, ICC/IF, IP applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Angiotensinogen AGT Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M02103)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
GGO-1
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Angiotensinogen
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M02103 is reactive to AGT in Human
Applications
M02103 is guaranteed for IP, IF, ICC, WB Boster Guarantee
Observed Molecular Weight
44 kDa
Calculated molecular weight
53.154kDa
Background of AGT
F-actin cross-linking protein which is thought to anchor actin to a variety of intracellular structures. This is a bundling protein. Probably involved in vesicular trafficking via its association with the CART complex. The CART complex is necessary for efficient transferrin receptor recycling but not for EGFR degradation.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Reconsitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:1000-1:2000
ICC/IF 1:50-1:200
IP 1:50
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of Angiotensinogen expression in 293T cell lysate.
Protein Target Info & Infographic
Gene/Protein Information For AGT (Source: Uniprot.org, NCBI)
Gene Name
AGT
Full Name
Angiotensinogen
Weight
53.154kDa
Superfamily
serpin family
Alternative Names
AGT angiotensinogen (serpin peptidase inhibitor, clade A, member 8); angiotensin I; angiotensin II; ANHU; SERPINA8 AGT ANHU, SERPINA8, hFLT1 angiotensinogen angiotensinogen|alpha-1 antiproteinase, antitrypsin|angiotensin I|angiotensin II|fetal-liver predominant transporter 1|pre-angiotensinogen|serine (or cysteine) proteinase inhibitor|serpin A8|serpin peptidase inhibitor, clade A, member 8
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on AGT, check out the AGT Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for AGT: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Angiotensinogen AGT Rabbit Monoclonal Antibody (M02103)
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No publications found for M02103
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Customer Reviews
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Customer Q&As
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3 Customer Q&As for Anti-Angiotensinogen AGT Rabbit Monoclonal Antibody
Question
Will M02103 anti-Angiotensinogen Rabbit Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2020-04-22
Answer
As indicated on the product datasheet, M02103 anti-Angiotensinogen Rabbit Monoclonal antibody as been tested on IP. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2020-04-22
Question
Is there a BSA free version of anti-Angiotensinogen Rabbit Monoclonal antibody M02103 available?
S. Zhao
Verified customer
Asked: 2019-11-26
Answer
Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-Angiotensinogen Rabbit Monoclonal antibody M02103 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-11-26
Question
Would anti-Angiotensinogen Rabbit Monoclonal antibody M02103 work for IP with serum?
Verified Customer
Verified customer
Asked: 2019-10-04
Answer
According to the expression profile of serum, AGT is highly expressed in serum. So, it is likely that anti-Angiotensinogen Rabbit Monoclonal antibody M02103 will work for IP with serum.
Boster Scientific Support
Answered: 2019-10-04