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Product Info Summary
|Reactive Species:||Human, Mouse, Rat|
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Boster Bio Anti-ANKLE1 Antibody (Catalog # A14094). Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
ANKLE1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-ANKLE1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A14094)
ANKLE1 Antibody is supplied in PBS containing 0.02% sodium azide.
ANKLE1 antibody was raised against a 15 amino acid synthetic peptide near the carboxy terminus of human ANKLE1. The immunogen is located within the last 50 amino acids of ANKLE1.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Two isoforms of ANKLE1 are known to exist. ANKLE1 antibody is predicted to not cross-react with ANKLE2
A14094 is reactive to ANKLE1 in Human, Mouse, Rat
A14094 is guaranteed for ELISA, WB Boster Guarantee
Background of LEM3
Ankyrins are membrane adaptor molecules that play important roles in coupling integral membrane proteins to the spectrin-based cytoskeleton network. ANKLE1 (Ankyrin repeat and LEM domain containing 1) is a member of ankyrin repeat and LEM domain containing family. It is a potential single-pass membrane protein that contains ankyrin repeats and a Lem domain. Ankyrin repeats mediate protein-protein interactions and are among the most common structural motifs in known proteins. While the function of this protein is still under investigation, its gene locus has been associated with susceptibility to ovarian and breast cancer.
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.
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Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.
ANKLE1 antibody can be used for detection of ANKLE1 by Western blot at 1 - 2 μg/mL. Antibody validated: Western Blot in human samples. All other applications and species not yet tested.
Validation Images & Assay Conditions
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Western blot analysis of ANKLE1 in 293 cell lysate with ANKLE1 antibody at (A) 1 and (B) 2 μg/mL.
Gene/Protein Information For ANKLE1 (Source: Uniprot.org, NCBI)
Ankyrin repeat and LEM domain-containing protein 1
ANKRD41; ankyrin repeat and LEM domain containing 1; ankyrin repeat and LEM domain-containing protein 1; FLJ39369; LEM domain containing 6; LEMD6; LEM-domain containing protein 3 ANKLE1 ANKRD41, LEM3, LEMD6 ankyrin repeat and LEM domain containing 1 ankyrin repeat and LEM domain-containing protein 1|LEM domain containing 6|LEM-domain containing 3|ankyrin repeat domain-containing protein 41*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".
For more info on ANKLE1, check out the ANKLE1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ANKLE1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected]
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