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Product Info Summary
| SKU: | A14094 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Chicken |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-ANKLE1 Antibody
SKU/Catalog Number
A14094
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-ANKLE1 Antibody (Catalog # A14094). Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
ANKLE1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-ANKLE1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A14094)
Host
Chicken
Contents
ANKLE1 Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgY
Immunogen
ANKLE1 antibody was raised against a 15 amino acid synthetic peptide near the carboxy terminus of human ANKLE1. The immunogen is located within the last 50 amino acids of ANKLE1.
Cross-reactivity
Two isoforms of ANKLE1 are known to exist. ANKLE1 antibody is predicted to not cross-react with ANKLE2
Reactive Species
A14094 is reactive to ANKLE1 in Human, Mouse, Rat
Calculated molecular weight
66.9 kDa
Background of ANKLE1
Ankyrins are membrane adaptor molecules that play important roles in coupling integral membrane proteins to the spectrin-based cytoskeleton network. ANKLE1 (Ankyrin repeat and LEM domain containing 1) is a member of ankyrin repeat and LEM domain containing family. It is a potential single-pass membrane protein that contains ankyrin repeats and a Lem domain. Ankyrin repeats mediate protein-protein interactions and are among the most common structural motifs in known proteins. While the function of this protein is still under investigation, its gene locus has been associated with susceptibility to ovarian and breast cancer.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A14094 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
ANKLE1 antibody can be used for detection of ANKLE1 by Western blot at 1 - 2 μg/mL.
Antibody validated: Western Blot in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of ANKLE1 in 293 cell lysate with ANKLE1 antibody at (A) 1 and (B) 2 μg/mL.
Specific Publications For Anti-ANKLE1 Antibody (A14094)
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