Product Info Summary
SKU: | A08527-1 |
---|---|
Size: | 100 μg/vial |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | ELISA, Flow Cytometry, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-AP2A2 Antibody Picoband™
SKU/Catalog Number
A08527-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-AP2A2 Antibody Picoband™ catalog # A08527-1. Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-AP2A2 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A08527-1)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human AP2A2 recombinant protein (Position: D686-K825).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A08527-1 is reactive to AP2A2 in Human, Mouse, Rat
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Observed Molecular Weight
104 kDa
Calculated molecular weight
Background of AP2A2
AP-2 complex subunit alpha-2 is a protein that in humans is encoded by the AP2A2 gene. The protein encoded by this gene is a subunit of the AP-2 adaptor protein complex, which is involved in linking lipid and protein membrane components with the clathrin lattice. This interaction supports the formation of clathrin-coated vesicles, and the encoded subunit aids in the process by binding polyphosphoinositide-containing lipids in the cell membrane.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A08527-1 is guaranteed for ELISA, Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.25 μg/ml, Human, Mouse, Rat
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml, Human
Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
Direct ELISA, 0.1-0.5 μg/ml, Human
Positive Control
Validation Images & Assay Conditions
![a08527 1 ap2a2 primary antibodies wb testing 1_1 a08527 1 ap2a2 primary antibodies wb testing 1_1](https://www.bosterbio.com/media/catalog/product/a/0/a08527-1-ap2a2-primary-antibodies-wb-testing-1_1.jpg)
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Figure 1. Western blot analysis of AP2A2 using anti-AP2A2 antibody (A08527-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human HepG2 whole cell lysates,
Lane 2: human HEK293 whole cell lysates,
Lane 3: human PC-3 whole cell lysates,
Lane 4: human SH-SY5Y whole cell lysates,
Lane 5: rat brain tissue lysates,
Lane 6: rat lung tissue lysates,
Lane 7: mouse brain tissue lysates,
Lane 8: mouse lung tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-AP2A2 antigen affinity purified polyclonal antibody (Catalog # A08527-1) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for AP2A2 at approximately 104 kDa. The expected band size for AP2A2 is at 104 kDa.
![a08527 1 ap2a2 primary antibodies ihc testing 2 a08527 1 ap2a2 primary antibodies ihc testing 2](https://www.bosterbio.com/media/catalog/product/a/0/a08527-1-ap2a2-primary-antibodies-ihc-testing-2.jpg)
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Figure 2. IHC analysis of AP2A2 using anti-AP2A2 antibody (A08527-1).
AP2A2 was detected in a paraffin-embedded section of human lymphoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-AP2A2 Antibody (A08527-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
![a08527 1 ap2a2 primary antibodies ihc testing 3 a08527 1 ap2a2 primary antibodies ihc testing 3](https://www.bosterbio.com/media/catalog/product/a/0/a08527-1-ap2a2-primary-antibodies-ihc-testing-3.jpg)
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Figure 3. IHC analysis of AP2A2 using anti-AP2A2 antibody (A08527-1).
AP2A2 was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-AP2A2 Antibody (A08527-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
![a08527 1 ap2a2 primary antibodies ihc testing 4 a08527 1 ap2a2 primary antibodies ihc testing 4](https://www.bosterbio.com/media/catalog/product/a/0/a08527-1-ap2a2-primary-antibodies-ihc-testing-4.jpg)
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Figure 4. IHC analysis of AP2A2 using anti-AP2A2 antibody (A08527-1).
AP2A2 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-AP2A2 Antibody (A08527-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
![a08527 1 ap2a2 primary antibodies ihc testing 5 a08527 1 ap2a2 primary antibodies ihc testing 5](https://www.bosterbio.com/media/catalog/product/a/0/a08527-1-ap2a2-primary-antibodies-ihc-testing-5.jpg)
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Figure 5. IHC analysis of AP2A2 using anti-AP2A2 antibody (A08527-1).
AP2A2 was detected in a paraffin-embedded section of human stomach cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-AP2A2 Antibody (A08527-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
![a08527 1 ap2a2 primary antibodies if testing 6 a08527 1 ap2a2 primary antibodies if testing 6](https://www.bosterbio.com/media/catalog/product/a/0/a08527-1-ap2a2-primary-antibodies-if-testing-6.jpg)
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Figure 6. IF analysis of AP2A2 using anti-AP2A2 antibody (A08527-1).
AP2A2 was detected in an immunocytochemical section of PC-3 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-AP2A2 Antibody (A08527-1) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
![a08527 1 ap2a2 primary antibodies fcm testing 7 a08527 1 ap2a2 primary antibodies fcm testing 7](https://www.bosterbio.com/media/catalog/product/a/0/a08527-1-ap2a2-primary-antibodies-fcm-testing-7.png)
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Figure 7. Flow Cytometry analysis of 293T cells using anti-AP2A2 antibody (A08527-1).
Overlay histogram showing 293T cells stained with A08527-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-AP2A2 Antibody (A08527-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Protein Target Info & Infographic
Gene/Protein Information For AP2A2 (Source: Uniprot.org, NCBI)
Gene Name
AP2A2
Full Name
AP-2 complex subunit alpha-2
Weight
Superfamily
adaptor complexes large subunit family
Alternative Names
100 kDa coated vesicle protein C; Adapter-related protein complex 2 alpha-2 subunit; adaptin, alpha B; Adaptor protein complex AP-2 subunit alpha-2; adaptor-related protein complex 2, alpha 2 subunit; alpha2-adaptin; Alpha-adaptin C; alpha-adaptin C; Huntingtin interacting protein J; AP-2 complex subunit alpha-2; Clathrin assembly protein complex 2 alpha-C large chain; clathrin-associated/assembly/adaptor protein, large, alpha 2; DKFZP564D1864; HIP9ADTAB; Huntingtin yeast partner J; Huntingtin-interacting protein 9; Huntingtin-interacting protein J; HYPJCLAPA2; KIAA0899HIP-9; Plasma membrane a AP2A2 ADTAB, CLAPA2, HIP-9, HIP9, HYPJ adaptor related protein complex 2 subunit alpha 2 AP-2 complex subunit alpha-2|100 kDa coated vesicle protein C|adapter-related protein complex 2 subunit alpha-2|adaptin, alpha B|adaptor related protein complex 2 alpha 2 subunit|alpha-adaptin C; Huntingtin interacting protein J|alpha2-adaptin|clathrin assembly protein complex 2 alpha-C large chain|clathrin-associated/assembly/adaptor protein, large, alpha 2|huntingtin yeast partner J|huntingtin-interacting protein 9|plasma membrane adaptor HA2/AP2 adaptin alpha C subunit
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on AP2A2, check out the AP2A2 Infographic
![AP2A2 infographic](/media/images/gene-infographic-example.jpg)
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for AP2A2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-AP2A2 Antibody Picoband™ (A08527-1)
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