Product Info Summary
SKU: | A07766 |
---|---|
Size: | 100 µl |
Reactive Species: | Human, Mouse |
Host: | Rabbit |
Application: | IHC, WB |
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Product info
Product Name
Anti-APBB2/Fe65L Antibody
SKU/Catalog Number
A07766
Size
100 µl
Form
Liquid
Description
Boster Bio Anti-APBB2/Fe65L Antibody (Catalog # A07766). Tested in WB, IHC applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-APBB2/Fe65L Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A07766)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from internal of human APBB2.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A07766 is reactive to APBB2 in Human, Mouse
Applications
A07766 is guaranteed for IHC, WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
83.374kDa
Background of APBB2
May modulate the internalization of beta-amyloid precursor protein.
The MGC Project Team; Genome Res. 14:2121-2127(2004).
Guenette S.Y., Proc. Natl. Acad. Sci. U.S.A. 93:10832-10837(1996).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blotting: 1:500~1:3000
Immunohistochemistry: 1:50~1:100
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of extracts from LOVO cells, using APBB2 antibody A07766.
Click image to see more details
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using APBB2 antibody A07766.
Protein Target Info & Infographic
Gene/Protein Information For APBB2 (Source: Uniprot.org, NCBI)
Gene Name
APBB2
Full Name
Amyloid-beta A4 precursor protein-binding family B member 2
Weight
83.374kDa
Alternative Names
amyloid beta (A4) precursor protein-binding, family B, member 2; amyloid beta A4 precursor protein-binding family B member 2; DKFZp434E033; FE65L1FE65LMGC35575; Fe65-like 1; Protein Fe65-like 1 APBB2 FE65L, FE65L1 amyloid beta precursor protein binding family B member 2 amyloid-beta A4 precursor protein-binding family B member 2|Fe65-like 1|protein Fe65-like 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on APBB2, check out the APBB2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for APBB2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-APBB2/Fe65L Antibody (A07766)
Hello CJ!
No publications found for A07766
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Customer Reviews
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Customer Q&As
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Find answers in Q&As, reviews.
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7 Customer Q&As for Anti-APBB2/Fe65L Antibody
Question
I was wanting to use your anti-APBB2/Fe65L antibody for IHC for human liver on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human liver identification?
Verified Customer
Verified customer
Asked: 2020-04-30
Answer
It shows on the product datasheet, A07766 anti-APBB2/Fe65L antibody has been tested for IHC, WB on human, mouse tissues. We have an innovator award program that if you test this antibody and show it works in human liver in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-04-30
Question
I see that the anti-APBB2/Fe65L antibody A07766 works with IHC, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2020-04-03
Answer
You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2020-04-03
Question
Please see the WB image, lot number and protocol we used for liver using anti-APBB2/Fe65L antibody A07766. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2020-03-09
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-03-09
Question
My question regarding product A07766, anti-APBB2/Fe65L antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2020-02-19
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A07766 anti-APBB2/Fe65L antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2020-02-19
Question
Will anti-APBB2/Fe65L antibody A07766 work on pig WB with amniotic fluid?
Verified Customer
Verified customer
Asked: 2018-06-18
Answer
Our lab technicians have not tested anti-APBB2/Fe65L antibody A07766 on pig. You can run a BLAST between pig and the immunogen sequence of anti-APBB2/Fe65L antibody A07766 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated pig samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in pig amniotic fluid in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-06-18
Question
Is a blocking peptide available for product anti-APBB2/Fe65L antibody (A07766)?
J. Kulkarni
Verified customer
Asked: 2015-04-21
Answer
We do provide the blocking peptide for product anti-APBB2/Fe65L antibody (A07766). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2015-04-21
Question
We are currently using anti-APBB2/Fe65L antibody A07766 for mouse tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human, mouse. Is it true that the antibody can work on monkey tissues as well?
C. Jones
Verified customer
Asked: 2014-10-24
Answer
The anti-APBB2/Fe65L antibody (A07766) has not been tested for cross reactivity specifically with monkey tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2014-10-24