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Product Info Summary
| SKU: | M00456-1 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IP, IF, ICC, WB |
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Product info
Product Name
Anti-beta 2 Microglobulin B2M Rabbit Monoclonal Antibody
SKU/Catalog Number
M00456-1
BM4209 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-beta 2 Microglobulin Rabbit Monoclonal Antibody catalog # M00456-1. Tested in WB, ICC/IF, IP applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-beta 2 Microglobulin B2M Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00456-1)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
CEA-2
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human beta 2 Microglobulin
Reactive Species
M00456-1 is reactive to B2M in Human, Mouse, Rat
Observed Molecular Weight
12 kDa
Calculated molecular weight
13.7 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00456-1 is guaranteed for IP, IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
ICC/IF 1:50-200
IP 1:20
Positive Control
WB: human U937 whole cell, human U251 whole cell, human THP-1 whole cell, human RT4 whole cell, rat liver tissue, rat RH35 whole cell, mouse liver tissue, mouse HEPA1-6 whole cell
Validation Images & Assay Conditions
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Western blot analysis of Beta-2 Microglobulin/B2M using anti-Beta-2 Microglobulin/B2M antibody (M00456-1).
Electrophoresis was performed on a 12% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human U937 whole cell lysates,
Lane 2: human U251 whole cell lysates,
Lane 3: human THP-1 whole cell lysates,
Lane 4: human RT4 whole cell lysates,
Lane 5: rat liver tissue lysates,
Lane 6: rat RH35 whole cell lysates,
Lane 7: mouse liver tissue lysates,
Lane 8: mouse HEPA1-6 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Beta-2 Microglobulin/B2M antigen affinity purified monoclonal antibody (M00456-1) at 1: 500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for Beta-2 Microglobulin/B2M at approximately 12 kDa. The expected band size for Beta-2 Microglobulin/B2M is at 14 kDa.
Specific Publications For Anti-beta 2 Microglobulin B2M Rabbit Monoclonal Antibody (M00456-1)
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Customer Reviews
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Customer Q&As
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15 Customer Q&As for Anti-beta 2 Microglobulin B2M Rabbit Monoclonal Antibody
Question
We are currently using anti-beta 2 Microglobulin Rabbit Monoclonal antibody M00456-1 for human tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on feline tissues as well?
Verified Customer
Verified customer
Asked: 2019-11-04
Answer
The anti-beta 2 Microglobulin Rabbit Monoclonal antibody (M00456-1) has not been tested for cross reactivity specifically with feline tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-11-04
Question
I would like to test anti-beta 2 Microglobulin Rabbit Monoclonal antibody M00456-1 on rat leukocyte for research purposes, then I may be interested in using anti-beta 2 Microglobulin Rabbit Monoclonal antibody M00456-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-10-15
Answer
The products we sell, including anti-beta 2 Microglobulin Rabbit Monoclonal antibody M00456-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-10-15
Question
Is this M00456-1 anti-beta 2 Microglobulin Rabbit Monoclonal antibody reactive to the isotypes of B2M?
Verified Customer
Verified customer
Asked: 2019-09-26
Answer
The immunogen of M00456-1 anti-beta 2 Microglobulin Rabbit Monoclonal antibody is A synthesized peptide derived from human beta 2 Microglobulin. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-09-26
Question
I have a question about product M00456-1, anti-beta 2 Microglobulin Rabbit Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-07-24
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M00456-1 anti-beta 2 Microglobulin Rabbit Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-07-24
Question
I was wanting to use your anti-beta 2 Microglobulin Rabbit Monoclonal antibody for IF for rat leukocyte on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for rat leukocyte identification?
Verified Customer
Verified customer
Asked: 2019-06-25
Answer
As indicated on the product datasheet, M00456-1 anti-beta 2 Microglobulin Rabbit Monoclonal antibody has been validated for IP, IF, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat leukocyte in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-06-25
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for leukocyte using anti-beta 2 Microglobulin Rabbit Monoclonal antibody M00456-1. Let me know if you need anything else.
C. Moore
Verified customer
Asked: 2018-10-09
Answer
I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-10-09
Question
Is a blocking peptide available for product anti-beta 2 Microglobulin Rabbit Monoclonal antibody (M00456-1)?
Verified Customer
Verified customer
Asked: 2017-12-11
Answer
We do provide the blocking peptide for product anti-beta 2 Microglobulin Rabbit Monoclonal antibody (M00456-1). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2017-12-11
Question
Do you have a BSA free version of anti-beta 2 Microglobulin Rabbit Monoclonal antibody M00456-1 available?
R. Miller
Verified customer
Asked: 2017-10-05
Answer
Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-beta 2 Microglobulin Rabbit Monoclonal antibody M00456-1 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2017-10-05
Question
We bought anti-beta 2 Microglobulin Rabbit Monoclonal antibody for WB on trachea last year. I am using rat, and We are going to use the antibody for IP next. I am interested in examining trachea as well as liver in our next experiment. Do you have any suggestion on which antibody would work the best for IP?
J. Gonzalez
Verified customer
Asked: 2017-10-02
Answer
I looked at the website and datasheets of our anti-beta 2 Microglobulin Rabbit Monoclonal antibody and it appears that M00456-1 has been tested on rat in both WB and IP. Thus M00456-1 should work for your application. Our Boster satisfaction guarantee will cover this product for IP in rat even if the specific tissue type has not been validated. We do have a comprehensive range of products for IP detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2017-10-02
Question
Our team were satisfied with the WB result of your anti-beta 2 Microglobulin Rabbit Monoclonal antibody. However we have been able to see positive staining in peripheral blood secreted using this antibody. Is that expected? Could you tell me where is B2M supposed to be expressed?
O. Krishna
Verified customer
Asked: 2017-07-04
Answer
From what I have seen in literature, peripheral blood does express B2M. Generally B2M expresses in secreted. Regarding which tissues have B2M expression, here are a few articles citing expression in various tissues:
Liver, Pubmed ID: 24275569
Macrophage, Pubmed ID: 25356553
Peripheral blood, Pubmed ID: 16108498
Plasma, Pubmed ID: 8084451
Retina, and Skin, Pubmed ID: 15489334
Tear, Pubmed ID: 25946035
Trachea, Pubmed ID: 14702039
Urine, Pubmed ID: 7554280
Boster Scientific Support
Answered: 2017-07-04
Question
We have seen staining in rat liver. Are there any suggestions? Is anti-beta 2 Microglobulin Rabbit Monoclonal antibody supposed to stain liver positively?
Verified Customer
Verified customer
Asked: 2017-06-09
Answer
From what I have seen in literature liver does express B2M. From what I have seen in Uniprot.org, B2M is expressed in leukocyte, peripheral blood, cervix carcinoma, dendritic cell, leukemia, trachea, retina skin, plasma, urine, tear, liver, macrophage, among other tissues. Regarding which tissues have B2M expression, here are a few articles citing expression in various tissues:
Liver, Pubmed ID: 24275569
Macrophage, Pubmed ID: 25356553
Peripheral blood, Pubmed ID: 16108498
Plasma, Pubmed ID: 8084451
Retina, and Skin, Pubmed ID: 15489334
Tear, Pubmed ID: 25946035
Trachea, Pubmed ID: 14702039
Urine, Pubmed ID: 7554280
Boster Scientific Support
Answered: 2017-06-09
Question
I see that the anti-beta 2 Microglobulin Rabbit Monoclonal antibody M00456-1 works with IF, what is the protocol used to produce the result images on the product page?
G. Huang
Verified customer
Asked: 2015-06-12
Answer
You can find protocols for IF on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2015-06-12
Question
Does M00456-1 anti-beta 2 Microglobulin Rabbit Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
S. Anderson
Verified customer
Asked: 2014-10-28
Answer
You can see on the product datasheet, M00456-1 anti-beta 2 Microglobulin Rabbit Monoclonal antibody as been validated on IF. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2014-10-28
Question
Here is the WB image, lot number and protocol we used for leukocyte using anti-beta 2 Microglobulin Rabbit Monoclonal antibody M00456-1. Please let me know if you require anything else.
C. Wu
Verified customer
Asked: 2013-10-09
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2013-10-09
Question
Would anti-beta 2 Microglobulin Rabbit Monoclonal antibody M00456-1 work for IF with leukocyte?
Z. Williams
Verified customer
Asked: 2013-09-16
Answer
According to the expression profile of leukocyte, B2M is highly expressed in leukocyte. So, it is likely that anti-beta 2 Microglobulin Rabbit Monoclonal antibody M00456-1 will work for IF with leukocyte.
Boster Scientific Support
Answered: 2013-09-16
