Anti-Apc2 ANAPC2 Antibody

Product Name

Anti-Apc2 ANAPC2 Antibody

View all ANAPC2 Antibodies

SKU/Catalog Number

A06153

Size

100uL

Form

Liquid (sterile filtered)

Description

Boster Bio Anti-Apc2 ANAPC2 Antibody (Catalog # A06153). Tested in IHC, WB applications. This antibody reacts with Human.

Storage & Handling

Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening.

Cite This Product

Anti-Apc2 ANAPC2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A06153)

Host

Rabbit

Contents

0.01% (w/v) Sodium Azide

Clonality

Polyclonal

Isotype

Antiserum

Immunogen

This antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids 810-822 of Human APC2 (C-terminal) coupled to KLH.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A06153 is reactive to ANAPC2 in Human

Applications

A06153 is guaranteed for IHC, WB Boster Guarantee

Observed Molecular Weight

39 kDa

Calculated molecular weight

93.828kDa

Background of ANAPC2

APC2, also known as Anaphase promoting complex subunit 2, APC2, Cyclosome subunit 2, and ANAPC2, is a component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G₁ phase of the cell cycle. The APC/cyclosome protein complex promotes metaphase-anaphase transition by ubiquitinating its specific substrates such as mitotic cyclins and anaphase inhibitors, which are subsequently degraded by the 26S proteasome. Biochemical studies have shown that the vertebrate APC contains at least eleven subunits. The composition of APC is highly conserved in organisms from yeast to humans. APC2 is a cullin family member that interacts through the cullin domain with ANAPC11 and UBCH10.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

ELISA: 1:2,000 - 1:10,000
IHC: User optimized
WB: 1:500 - 1:1,000

Validation Images & Assay Conditions

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Most modifiers mature by proteolytic processing from inactive precursors (a; amino acid). Arrowheads point to the cleavage sites. Ubiquitin is expressed either as polyubiquitin or as a fusion with ribosomal proteins. Conjugation requires activating (E1) and conjugating (E2) enzymes that form thiolesters (S) with the modifiers. Modification of cullins by RUB involves SCF(SKP1/cullin-1/F-box protein) /CBC(cullin-2/elongin B/elonginC) -like E3 enzymes that are also involved in ubiquitination. In contrast to ubiquitin, the UBLs do not seem to form multi-UBL chains. UCRP(ISG15) resembles two ubiquitin moieties linked head-to-tail. Whether HUB1 functions as a modifier is currently unclear. APG12 and URM1 are distinct from the other modifiers because they are unrelated in sequence to ubiquitin.Data contributed by S.Jentsch.

Gene/Protein Information For ANAPC2 (Source: Uniprot.org, NCBI)

Alternative Names

anaphase promoting complex subunit 2; anaphase-promoting complex subunit 2; APC2KIAA1406Cyclosome subunit 2; RP11-350O14.5 ANAPC2 APC2 anaphase promoting complex subunit 2 anaphase-promoting complex subunit 2|cyclosome subunit 2

For more info on ANAPC2, check out the ANAPC2 Infographic

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In this infographic, you will see the following information for ANAPC2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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