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Product Info Summary
| SKU: | A04859 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IHC-P, WB |
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Product info
Product Name
Anti-APH1 Antibody
SKU/Catalog Number
A04859
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-APH1 Antibody (Catalog # A04859). Tested in ELISA, WB, IHC-P applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
APH1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-APH1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A04859)
Host
Rabbit
Contents
APH1 Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
APH1 antibody was raised against a 18 amino acid synthetic peptide from near the center of human APH1. The immunogen is located within amino acids 80 - 130 of APH1.
Reactive Species
A04859 is reactive to APH1A in Human, Mouse, Rat
Observed Molecular Weight
68 kDa
Calculated molecular weight
29.0 kDa
Background of APH1A
APH1 was initially identified as a component of the Notch pathway in C. elegans. Along with nicastrin, PEN2, and presenilin-1 APH1 is an essential component of the gamma-secretase complex which cleave the amyloid precursor protein (APP) at what are known as the gamma- and epsilon-sites and can lead to the accumulation of the Amyloid beta peptide (Abeta) cleavage product that is associated with Alzheimer's disease. APH1 exists in at least three distinct isoforms with APH1a as the principal isoform present in the gamma-secretase complex. Mice deficient in this isoform, but not the other two, were lethal at E10.5, with impaired vascular and neural development observed.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A04859 is guaranteed for ELISA, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
APH1 antibody can be used for detection of APH1 by Western blot at 0.5 - 1 μg/mL. Despite its predicted molecular weight, APH1 protein often migrates at aberrant locations in SDS-PAGE. Antibody can also be used for immunohistochemistry starting at 5 μg/mL.
Antibody validated: Western Blot in mouse samples and Immunohistochemistry in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of APH1 in RAW264.7 cell lysate with APH1 antibody at (A) 1 and (B) 2 μg/mL.
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Immunohistochemistry of APH1 in human brain tissue with APH1 antibody at 5 μg/mL.
Specific Publications For Anti-APH1 Antibody (A04859)
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