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Product Info Summary
| SKU: | A03444-2 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, Flow Cytometry, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-ApoER2/LRP8 Antibody Picoband®
SKU/Catalog Number
A03444-2
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-ApoER2/LRP8 Antibody Picoband® catalog # A03444-2. Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance. ApoER2/LRP8 participates in transmitting extracellular Reelin signals to intracellular processes by binding the adaptor DAB1 via its cytoplasmic tail (well-established). Assay context: primary antibody reactive with human/mouse/rat, validated for ELISA, flow cytometry, IF, IHC, ICC, and WB on the product page. In neurobiology and receptor-trafficking studies (putative), LRP8 distribution can be mapped by IHC or IF/ICC, and signaling consequences can be evaluated in engineered systems using reporter cell lines (study-dependent); innate immune context in the same specimens is sometimes assessed with myeloid markers such as TREM2 (putative).
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-ApoER2/LRP8 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03444-2)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.01mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human ApoER2/LRP8 recombinant protein (Position: R444-D960).
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A03444-2 is reactive to LRP8 in Human, Mouse, Rat
Observed Molecular Weight
106 kDa
Calculated molecular weight
105.6 kDa
Background of LRP8
This gene encodes a member of the low density lipoprotein receptor (LDLR) family. Low density lipoprotein receptors are cell surface proteins that play roles in both signal transduction and receptor-mediated endocytosis of specific ligands for lysosomal degradation. The encoded protein plays a critical role in the migration of neurons during development by mediating Reelin signaling, and also functions as a receptor for the cholesterol transport protein apolipoprotein E. Expression of this gene may be a marker for major depressive disorder. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A03444-2 is guaranteed for ELISA, Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.25μg/ml, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 4μg/ml, Human
Flow Cytometry (Fixed), 1-3μg/1x106 cells, Human
ELISA, 0.1-0.5μg/ml, -
Positive Control
WB: human placenta tissue, human U87 whole cell, human A549 whole cell, human U937 whole cell, human HL-60 whole cell, human A431 whole cell, human Hela whole cell, rat testis tissue, rat thymus tissue, rat spleen tissue, rat heart tissue, mouse testis tissue, mouse thymus tissue, mouse heart tissue, mouse Raw2647 whole cell
IHC: human lung cancer tissue, human mammary cancer tissue, human rectal cancer tissue, mouse intestine tissue, rat intestine tissue
ICC/IF: HepG2 cell
FCM: U87 cell, HL-60 cell
Validation Images & Assay Conditions
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Western blot analysis of ApoER2/LRP8 using anti-ApoER2/LRP8 antibody (A03444-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human placenta tissue lysates,
Lane 2: human U87 whole cell lysates,
Lane 3: human A549 whole cell lysates,
Lane 4: human U937 whole cell lysates,
Lane 5: human HL-60 whole cell lysates,
Lane 6: human A431 whole cell lysates,
Lane 7: human Hela whole cell lysates,
Lane 8: rat testis tissue lysates,
Lane 9: rat thymus tissue lysates,
Lane 10: rat spleen tissue lysates,
Lane 11: rat heart tissue lysates,
Lane 12: mouse testis tissue lysates,
Lane 13: mouse thymus tissue lysates,
Lane 14: mouse heart tissue lysates,
Lane 15: mouse Raw264.7 whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ApoER2/LRP8 antigen affinity purified polyclonal antibody (Catalog # A03444-2) at 0.25 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ApoER2/LRP8 at approximately 106KD. The expected band size for ApoER2/LRP8 is at 106KD.
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IHC analysis of ApoER2/LRP8 using anti-ApoER2/LRP8 antibody (A03444-2).
ApoER2/LRP8 was detected in paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-ApoER2/LRP8 Antibody (A03444-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
IHC analysis of ApoER2/LRP8 using anti-ApoER2/LRP8 antibody (A03444-2).
ApoER2/LRP8 was detected in paraffin-embedded section of human mammary cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-ApoER2/LRP8 Antibody (A03444-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
IHC analysis of ApoER2/LRP8 using anti-ApoER2/LRP8 antibody (A03444-2).
ApoER2/LRP8 was detected in paraffin-embedded section of human rectal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-ApoER2/LRP8 Antibody (A03444-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
IHC analysis of ApoER2/LRP8 using anti-ApoER2/LRP8 antibody (A03444-2).
ApoER2/LRP8 was detected in paraffin-embedded section of mouse intestine tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-ApoER2/LRP8 Antibody (A03444-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
IHC analysis of ApoER2/LRP8 using anti-ApoER2/LRP8 antibody (A03444-2).
ApoER2/LRP8 was detected in paraffin-embedded section of rat intestine tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-ApoER2/LRP8 Antibody (A03444-2) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
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Flow Cytometry analysis of U87 cells using anti-ApoER2/LRP8 antibody (A03444-2).
Overlay histogram showing U87 cells stained with A03444-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ApoER2/LRP8 Antibody (A03444-2, 1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
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Flow Cytometry analysis of HL-60 cells using anti-ApoER2/LRP8 antibody (A03444-2).
Overlay histogram showing HL-60 cells stained with A03444-2 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ApoER2/LRP8 Antibody (A03444-2, 1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
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IF analysis of ApoER2/LRP8 using anti-ApoER2/LRP8 antibody (A03444-2).
ApoER2/LRP8 was detected in immunocytochemical section of HepG2 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 4μg/mL rabbit anti-ApoER2/LRP8 Antibody (A03444-2) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
Specific Publications For Anti-ApoER2/LRP8 Antibody Picoband® (A03444-2)
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