Product Info Summary
SKU: | M01049 |
---|---|
Size: | 100ul |
Reactive Species: | Human, Mouse, Rat |
Host: | Mouse |
Application: | IF, IHC, ICC, WB |
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Product info
Product Name
Anti-Aquaporin 4 AQP4 Monoclonal Antibody
View all Aquaporin-4 Antibodies
SKU/Catalog Number
M01049
Size
100ul
Form
Liquid
Description
Boster Bio Anti-Aquaporin 4 AQP4 Monoclonal Antibody catalog # M01049. Tested in IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Aquaporin 4 AQP4 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01049)
Host
Mouse
Contents
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Monoclonal
Clone Number
4H1
Isotype
IgG
Immunogen
Synthetic peptide of Aquaporin 4
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
M01049 is reactive to AQP4 in Human, Mouse, Rat
Applications
M01049 is guaranteed for IF, IHC, ICC, WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
34.83kDa
Background of Aquaporin-4
TRPC6, also known as TRP6, short transient receptor potential channel 6 and transient receptor potential cation channel subfamily C member 6, is thought to form a receptor-activated non-selective calcium permeant cation channel. TRPC6 is probably operated by a phosphatidylinositol second messenger system activated by receptor tyrosine kinases or G-protein coupled receptors. It is activated by diacylglycerol (DAG) in a membrane-delimited fashion, independently of protein kinase C and may not to be activated by intracellular calcium store depletion. Defects in this gene are a cause of focal segmental glomerulosclerosis (FSGS). Expression of this protein has been reported in tissues such as placenta, lung, spleen, ovary, small intestine, and renal podocytes. Immunohistochemistry studies using polyclonal antibodies to this target have shown moderate to strong staining in cell types such as neurons, breast, respiratory, squamous and prostate epithelium, epidermis, placental trophoblasts, dendritic cells, and subsets of immune cells, and faint to moderate staining of adrenal, colon, ganglion cells, hepatocytes, heart, and testis.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:1000
IF 1:100-200
IHC 1:50-300
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of lysates from 1) Hela, 2) Mouse Heart tissue, 3) Rat Heart Tissue cells, (Green) primary antibody was diluted at 1:1000, 4°C over night, secondary antibody (cat: (NA) was diluted at 1:10000, 37°C 1hour. (Red) GAPDH Polyclonal Antibody (cat: (STJ97090) antibody was diluted at 1:5000 as loading control, 4°C over night, secondary antibody (cat: (NA) was diluted at 1:10000, 37°C 1hour.
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IF analysis of Hela with antibody (Left) and DAPI (Right) diluted at 1:100.
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Western blot analysis of 1) Hela, 2) Mouse Heart tissue, 3) Rat Heart Tissue, diluted at 1:2000.
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Immunofluorescence analysis of Mouse-brain tissue. 1, Aquaporin 4 monoclonal antibody (4H1) (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
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Immunofluorescence analysis of Human-appendix tissue. 1, Aquaporin 4 monoclonal antibody (4H1) (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B
Click image to see more details
Immunohistochemical analysis of paraffin-embedded Human-liver-cancer tissue. 1, Aquaporin 4 monoclonal antibody (4H1) was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
Protein Target Info & Infographic
Gene/Protein Information For AQP4 (Source: Uniprot.org, NCBI)
Gene Name
AQP4
Full Name
Aquaporin-4
Weight
34.83kDa
Superfamily
MIP/aquaporin (TC 1.A.8) family
Alternative Names
aquaporin 4; MGC22454 AQP4 MIWC, WCH4 aquaporin 4 aquaporin-4|aquaporin type4|mercurial-insensitive water channel
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on AQP4, check out the AQP4 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for AQP4: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Aquaporin 4 AQP4 Monoclonal Antibody (M01049)
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No publications found for M01049
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