Anti-Aquaporin 5/AQP5 Antibody
Rabbit IgG polyclonal antibody for Aquaporin-5(AQP5) detection. Tested with WB, IHC-P in Mouse;Rat. Cited in 2 publication(s).
Anti-Aquaporin 5/AQP5 Antibody Info At A Glance
|Product Name||Anti-Aquaporin 5/AQP5 Antibody
See all Aquaporin-5 primary antibodies, ELISA kits and proteins
|Storage & Handling||At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.|
|Description||Polyclonal antibody for AQP5 detection. Host: Rabbit.Size: 100μg/vial. Tested applications: IHC-P. Reactive species: Mouse. AQP5 information: Molecular Weight: 28274 MW; Subcellular Localization: Membrane; Multi-pass membrane protein.|
|Cite This Product||Anti-Aquaporin 5/AQP5 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1230)|
|Similar Products From
|Anti-Aquaporin 5/AQP5 Antibody may replace the following items: sc 28628|sc 9890|sc 9891|sc 514022.|
*Our Boster Guarantee covers the use of this product in the above tested applications.
*Innovating Scientists reward: if you test this antibody on a species or application not listed above and share with us your results, we will provide you a full credit to purchase Boster products.
|Related Products||Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P).
*Blocking peptide can be purchased at $50. Contact us for more information.
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion all year round.
|Immunogen||A synthetic peptide corresponding to a sequence at the C-terminus of mouse AQP5(246-265aa EEDWEDHREERKKTIELTAH), identical to the related rat sequence.|
|Cross Reactivity||No cross reactivity with other proteins|
Gene/Protein Basic Information For Aqp5 (Source: Uniprot.org, NCBI)
|NCBI Gene Id||11830|
|Species Of This Entry||Mouse|
|Superfamily||MIP/aquaporin (TC 1.A.8) family|
|Alternative Names||Aquaporin-5|AQP-5; aquaporin 5; aquaporin-5|
|Post Tranlational Modifications||Phosphorylation; Cleavage; Demethylation; Methylation; Glycosylation; Dephosphorylation; Biotinylation; Reduction; Acetylation|
|Gene Location||15 F1|15 56.13 cM, on Chromasome 15, gene sequence:|
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
Ontology For Aqp5 (Source: Uniprot.org, NCBI)
|Protein Function||Forms a water-specific channel. Implicated in the generation of saliva, tears, and pulmonary secretions. Required for TRPV4 activation by hypotonicity (PubMed:16571723). Together with TRPV4, controls regulatory volume decrease in salivary epithelial cells (PubMed:16571723).|
|Tissue Specificity||Expressed in salivary glands (at protein level).|
|Research Areas||Cancer; Signal Transduction|
|Related Diseases||Salivary Gland Diseases; Edema; Lung Injury; Sjogren's Syndrome; Malignant Neoplasms; Inflammation; Neoplasms; Corneal Diseases; Pulmonary Edema; Xerostomia; Acute Lung Injury; Neoplasm Metastasis; Lung Diseases; Diabetes Mellitus; Carcinoma; Fibrosis; Mucinous Adenocarcinoma; Hyperplasia; Pneumonia|
|Related Pathways||Transport; Secretion; Localization; Water Transport; Fluid Transport; Water Homeostasis; Cell Proliferation; Pathogenesis; Lung Development; Saliva Secretion; Cell Differentiation; Cell Migration; Cellular Localization; Transdifferentiation; Exocytosis; Reverse Transcription; Cell Growth; Epithelial Cell Differentiation; Gland Development; Regeneration|
|Background||Aquaporin 5, also known as AQP5, is a water channel protein. The aquaporins(AQPs) are a family of more than 10 homologous water transporting proteins expressed in many mammalian epithelia and endothelia. At least five AQPs are expressed in the eye: AQP0(MIP) in lens fiber, AQP1 in cornea endothelium, ciliary and lens epithelia and trabecular meshwork, AQP3 in conjunctiva, AQP4 in ciliary epithelium and retinal Müller cells, and AQP5 in corneal and lacrimal gland epithelia. Among the seven human aquaporins cloned to date(AQPs 0-6), genes encoding the four most closely related aquaporins(AQP0, AQP2, AQP5, and AQP6) have been mapped to chromosome band 12q13, suggesting an aquaporin family gene cluster at this locus. Aquaporin 5 plays a role in the generation of saliva, tears and pulmonary secretions.|
|Scientific References||PMID: 10337625 by Krane C.M., et al. Cloning and characterization of murine Aqp5: evidence for a conserved aquaporin gene cluster.|
PMID: 10400615 by Ma T., et al. Defective secretion of saliva in transgenic mice lacking aquaporin-5 water channels.
Our Boster Quality Guarantee for Anti-Aquaporin 5/AQP5 Antibody covers its use in the following applications.Immunohistochemistry(Paraffin-embedded Section), 0.5-1μg/ml, Rat, Mouse, By Heat
Western blot, 0.1-0.5μg/ml, Rat, Mouse
*The recommended dilution ratios/concentrations are for reference only and optimal dilutions/concentrations should be determined by the end user.
Working concentration: 0.5μg/ml
Lane 1: Rat Lung Tissue Lysate
Lane 2: Rat Testis Tissue Lysate
IHC(P): Rat Lung Tissue Lysate
Working concentration: 1μg/ml
Boster provides comprehensive technical information for WB, IHC/IF/ICC, Flow Cytometry sample preparation protocols, assay protocols, troubleshooting tips and assay optimization tips.
- Western Blottting Resource Center
- Immunohistochemistry Resource Center
- Flow Cytometry Technical Resource Center
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Total number of citations: 2
|Trinucleotide repeat containing 6c (TNRC6c) is essential for microvascular maturation during distal airspace sacculation in the developing lung||PubMed ID 28811219|
|Hyperosmotic stress induces cisplatin sensitivity in ovarian cancer cells by stimulating aquaporin-5 expression||PubMed ID 26668595|
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Note: sample size takes longer to process, average 5-7 days.
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