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Product Info Summary
| SKU: | A03825 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-ARP Antibody
SKU/Catalog Number
A03825
Size
100μl
Form
Liquid
Description
Boster Bio Anti-ARP Antibody catalog # A03825. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-ARP Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03825)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from the Internal region of human ARP.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A03825 is reactive to MANF in Human, Mouse, Rat
Applications
A03825 is guaranteed for IHC, WB Boster Guarantee
Observed Molecular Weight
39 kDa
Calculated molecular weight
20.7kDa
Background of MANF
Hypoxia-inducible factor 3 alpha, also called MOP7, is a protein that in humans is encoded by the HIF3A gene. HIF3A is mapped to 19q13.32. This gene involved in adaptive response to hypoxia. HIF3A suppresses hypoxia-inducible expression of HIF1A and EPAS1. It binds to core DNA sequence 5'-TACGTG-3' within the hypoxia response element (HRE) of target gene promoters. The complex HIF3A-ARNT activates the transcription of reporter genes driven by HRE. This gene functions as an inhibitor of angiogenesis in the cornea.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB, 1:500-1:2000
IHC-P, 1:100-1:300
Validation Images & Assay Conditions
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Immunohistochemistry (IHC) analysis of paraffin-embedded Rat Brain, antibody was diluted at 1:100.
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Western Blot (WB) analysis of 293 cells using ARP Polyclonal antibody.
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Immunohistochemistry (IHC) analysis of paraffin-embedded Rat Brain, antibody was diluted at 1:100.
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Immunohistochemistry (IHC) analysis of paraffin-embedded Mouse Brain, antibody was diluted at 1:100.
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Immunohistochemistry (IHC) analysis of paraffin-embedded Rat Brain, antibody was diluted at 1:100.
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Immunohistochemistry (IHC) analysis of paraffin-embedded Mouse Brain, antibody was diluted at 1:100.
Protein Target Info & Infographic
Gene/Protein Information For MANF (Source: Uniprot.org, NCBI)
Gene Name
MANF
Full Name
Mesencephalic astrocyte-derived neurotrophic factor
Weight
20.7kDa
Superfamily
ARMET family
Alternative Names
Arginine-Rich Protein; arginine-rich, mutated in early stage tumors; ARMET; ARP; MANF; mesencephalic astrocyte-derived neurotrophic factor; Protein ARMET MANF ARMET, ARP mesencephalic astrocyte derived neurotrophic factor mesencephalic astrocyte-derived neurotrophic factor|arginine-rich, mutated in early stage tumors
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on MANF, check out the MANF Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for MANF: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-ARP Antibody (A03825)
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No publications found for A03825
*Do you have publications using this product? Share with us and receive a reward. Ask us for more details.
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
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- Scientific Literature Resources
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Customer Reviews
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*The first user to submit a review for a product is eligible for Boster's Innovating Scientists Reward, which gives product credits. This is in addition to the gift card reward.
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
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6 Customer Q&As for Anti-ARP Antibody
Question
We are currently using anti-ARP antibody A03825 for mouse tissue, and we are satisfied with the IHC results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on goat tissues as well?
Verified Customer
Verified customer
Asked: 2020-04-27
Answer
The anti-ARP antibody (A03825) has not been validated for cross reactivity specifically with goat tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-04-27
Question
Do you have a BSA free version of anti-ARP antibody A03825 available?
Verified Customer
Verified customer
Asked: 2019-11-13
Answer
Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-ARP antibody A03825 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-11-13
Question
My lab would like to test anti-ARP antibody A03825 on mouse liver for research purposes, then I may be interested in using anti-ARP antibody A03825 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-10-24
Answer
The products we sell, including anti-ARP antibody A03825, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-10-24
Question
I was wanting to use your anti-ARP antibody for WB for mouse liver on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for mouse liver identification?
Verified Customer
Verified customer
Asked: 2019-08-14
Answer
It shows on the product datasheet, A03825 anti-ARP antibody has been validated for IHC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in mouse liver in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-08-14
Question
Does anti-ARP antibody A03825 work on feline IHC with colon eye?
Verified Customer
Verified customer
Asked: 2019-04-18
Answer
Our lab technicians have not validated anti-ARP antibody A03825 on feline. You can run a BLAST between feline and the immunogen sequence of anti-ARP antibody A03825 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated feline samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in feline colon eye in IHC, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-04-18
Question
I have attached the WB image, lot number and protocol we used for liver using anti-ARP antibody A03825. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2018-08-21
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-08-21
