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Product Info Summary
| SKU: | M02096-1 |
|---|---|
| Size: | 50 µl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IF, WB |
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Product info
Product Name
Anti-ARPC5 Antibody (Center)
SKU/Catalog Number
M02096-1
Size
50 µl
Description
Boster Bio Anti-ARPC5 Antibody (Center) (Catalog # M02096-1). Tested in WB, IF application(s). This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Cite This Product
Anti-ARPC5 Antibody (Center) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M02096-1)
Host
Rabbit
Contents
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
This ARPC5 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 67-101 amino acids from the Central region of human ARPC5.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M02096-1 is reactive to ARPC5 in Human, Mouse, Rat
Applications
M02096-1 is guaranteed for IF, WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
16.32kDa
Background of ARPC5
Functions as component of the Arp2/3 complex which is involved in regulation of actin polymerization and together with an activating nucleation-promoting factor (NPF) mediates the formation of branched actin networks.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
IF: 1:25
WB: 1:2000
Validation Images & Assay Conditions
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Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized U-2 OS (human osteosarcoma cell line) cells labeling ARPC5 with M02096-1 at 1/25 dilution, followed by Dylight® 488-conjugated goat anti-rabbit IgG secondary antibody at 1/200 dilution (green). Immunofluorescence image showing cytoplasm and weak nucleus staining on U-2 OS cell line. Cytoplasmic actin is detected with Dylight® 554 Phalloidin at 1/100 dilution (red).The nuclear counter stain is DAPI (blue).
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All lanes : Anti-ARPC5 Antibody (Center) at 1:2000 dilution
Lane 1: THP-1 whole cell lysate
Lane 2: HL-60 whole cell lysate
Lane 3: Hela whole cell lysate
Lane 4: MCF-7 whole cell lysate
Lane 5: Human spleen lysate
Lane 6: Mouse brain lysate
Lane 7: Rat brain lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 16 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
Protein Target Info & Infographic
Gene/Protein Information For ARPC5 (Source: Uniprot.org, NCBI)
Gene Name
ARPC5
Full Name
Actin-related protein 2/3 complex subunit 5
Weight
16.32kDa
Superfamily
ARPC5 family
Alternative Names
actin related protein 2/3 complex, subunit 5, 16kDa; ARC16subunit 5 (16 kD); MGC88523 ARPC5 ARC16, dJ127C7.3, p16-Arc actin related protein 2/3 complex subunit 5 actin-related protein 2/3 complex subunit 5|Arp2/3 protein complex subunit p16|actin related protein 2/3 complex, subunit 5, 16kDa|arp2/3 complex 16 kDa subunit
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ARPC5, check out the ARPC5 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ARPC5: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-ARPC5 Antibody (Center) (M02096-1)
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