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Product Info Summary
| SKU: | A10294-2 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Monkey |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-ARSE/ASE/ARSL Antibody Picoband™
SKU/Catalog Number
A10294-2
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-ARSE/ASE/ARSL Antibody Picoband™ catalog # A10294-2. Tested in WB applications. This antibody reacts with Human, Monkey.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-ARSE/ASE/ARSL Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A10294-2)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.01mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human ARSE/ASE/ARSL.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A10294-2 is reactive to ARSL in Human, Monkey
Applications
A10294-2 is guaranteed for WB Boster Guarantee
Observed Molecular Weight
66 kDa
Calculated molecular weight
65.669kDa
Background of ARSL
Arylsulfatase E, also known as ARSE, is an enzyme that, in humans, is encoded by the ARSE gene. Arylsulfatase E is a member of the sulfatase family. It is glycosylated postranslationally and localized to the golgi apparatus. Sulfatases are essential for the correct composition of bone and cartilage matrix. X-linked chondrodysplasia punctata, a disease characterized by abnormalities in cartilage and bone development, has been linked to mutations in this gene. Alternative splicing results in multiple transcript variants. A pseudogene related to this gene is located on the Y chromosome.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Reconsitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5μg/ml, Human, Monkey
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of ARSE/ASE/ARSL using anti-ARSE/ASE/ARSL antibody (A10294-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human Hek293 whole cell lysates,
Lane 2: monkey Cos-7 whole cell lysates,
Lane 3: human HK-2 whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ARSE/ASE/ARSL antigen affinity purified polyclonal antibody (Catalog # A10294-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ARSE/ASE/ARSL at approximately 66KD. The expected band size for ARSE/ASE/ARSL is at 66KD.
Protein Target Info & Infographic
Gene/Protein Information For ARSL (Source: Uniprot.org, NCBI)
Gene Name
ARSL
Full Name
Arylsulfatase L
Weight
65.669kDa
Superfamily
sulfatase family
Alternative Names
Arylsulfatase L ARSL ARSE, ASE, CDPX, CDPX1, CDPXR arylsulfatase L arylsulfatase L|arylsulfatase E (chondrodysplasia punctata 1)
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ARSL, check out the ARSL Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ARSL: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-ARSE/ASE/ARSL Antibody Picoband™ (A10294-2)
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No publications found for A10294-2
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Customer Reviews
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