Anti-ASC1/TRIP4 Antibody Picoband™

Product Name

Anti-ASC1/TRIP4 Antibody Picoband™

View all ASC1 Antibodies

SKU/Catalog Number

A07762-2

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-ASC1/TRIP4 Antibody Picoband™ catalog # A07762-2. Tested in ELISA, WB applications. This antibody reacts with Human.

Storage & Handling

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Cite This Product

Anti-ASC1/TRIP4 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A07762-2)

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived human ASC1/TRIP4 recombinant protein (Position: Q158-D563).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A07762-2 is reactive to TRIP4 in Human

Applications

A07762-2 is guaranteed for ELISA, WB Boster Guarantee

Observed Molecular Weight

68 kDa

Calculated molecular weight

66.146kDa

Background of ASC1

Activating signal cointegrator 1 is a protein that in humans is encoded by the TRIP4 gene. This gene encodes a subunit of the tetrameric nuclear activating signal cointegrator 1 (ASC-1) complex, which associates with transcriptional coactivators, nuclear receptors and basal transcription factors to facilitate nuclear receptors-mediated transcription. This protein is localized in the nucleus and contains an E1A-type zinc finger domain, which mediates interaction with transcriptional coactivators and ligand-bound nuclear receptors, such as thyroid hormone receptor and retinoid X receptor alpha, but not glucocorticoid receptor. Mutations in this gene are associated with spinal muscular atrophy with congenital bone fractures-1 (SMABF1).

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.25-0.5 µg/ml, Human
Direct ELISA, 0.1-0.5 µg/ml, Human

Validation Images & Assay Conditions

Click image to see more details

Figure 1. Western blot analysis of ASC1/TRIP4 using anti-ASC1/TRIP4 antibody (A07762-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human Jurkat whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ASC1/TRIP4 antigen affinity purified polyclonal antibody (Catalog # A07762-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ASC1/TRIP4 at approximately 68 kDa. The expected band size for ASC1/TRIP4 is at 66 kDa.

Gene/Protein Information For TRIP4 (Source: Uniprot.org, NCBI)

Alternative Names

activating signal cointegrator 1; ASC-1; HsT17391; thyroid hormone receptor interactor 4; thyroid receptor interacting protein 4; Thyroid receptor-interacting protein 4; TR-interacting protein 4; TRIP-4 TRIP4 ASC-1, ASC1, HsT17391, MDCDC, SMABF1, ZC2HC5 thyroid hormone receptor interactor 4 activating signal cointegrator 1|TR-interacting protein 4|TRIP-4|thyroid receptor-interacting protein 4|zinc finger, C2HC5-type

For more info on TRIP4, check out the TRIP4 Infographic

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In this infographic, you will see the following information for TRIP4: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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