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- Table of Contents
Designing a Western blot for Histone chaperone ASF1B? The guide has the expected band size, antibodies backed by real blot images, the controls to run alongside, and protocols taken from published papers.
Open the ASF1B Western Blot GuideDesigning an IHC experiment for Histone chaperone ASF1B? The guide has the expected staining pattern, antigen retrieval and fixation, antibodies backed by real IHC images, the tissue controls to run alongside, and protocols taken from published papers.
Open the ASF1B IHC GuideFacts about Histone chaperone ASF1B.
Does not take part in replication-independent nucleosome deposition which is mediated by ASF1A and HIRA. Required for spermatogenesis.
| Human | |
|---|---|
| Gene Name: | ASF1B |
| Uniprot: | Q9NVP2 |
| Entrez: | 55723 |

| Belongs to: |
|---|
| ASF1 family |

ASF1 silencing function 1 homolog B (S. cerevisiae); CCG1-interacting factor A-II; CIA-II; FLJ10604; hAsf1; hAsf1b; hCIA-II; histone chaperone ASF1B; silencing function protein 1 homolog B
Mass (kDA):
22.434 kDA

| Human | |
|---|---|
| Location: | 19p13.12 |
| Sequence: | 19; NC_000019.10 (14119512..14136589, complement) |
Highly expressed in testis and at lower levels in colon, small intestine and thymus.
Nucleus.





PMID: 11470414 by Sillje H.H.W., et al. Identification of human Asf1 chromatin assembly factors as substrates of Tousled-like kinases.
PMID: 12842904 by Umehara T., et al. Transcription initiation factor IID-interactive histone chaperone CIA-II implicated in mammalian spermatogenesis.