Anti-ATF5 Rabbit Monoclonal Antibody

Product Name

Anti-ATF5 Rabbit Monoclonal Antibody

View all ATF5 Antibodies

SKU/Catalog Number

M02792

Size

100 μl

Form

Liquid

Description

Boster Bio Anti-ATF5 Rabbit Monoclonal Antibody catalog # M02792. Tested in WB, IHC, ICC/IF, IP applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-ATF5 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M02792)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Clonality

Monoclonal

Clone Number

HDD-1

Isotype

Rabbit IgG

Immunogen

A synthesized peptide derived from human ATF5

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

M02792 is reactive to ATF5 in Human, Mouse, Rat

Applications

M02792 is guaranteed for IP, IF, IHC, ICC, WB Boster Guarantee

Observed Molecular Weight

48 kDa

Calculated molecular weight

30.674kDa

Background of ATF5

F-actin cross-linking protein which is thought to anchor actin to a variety of intracellular structures. This is a bundling protein. Probably involved in vesicular trafficking via its association with the CART complex. The CART complex is necessary for efficient transferrin receptor recycling but not for EGFR degradation.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

View more details

Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.

Submit A Review

Reconsitution

Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB 1:1000-1:2000
IHC 1:100-1:500
ICC/IF 1:50-1:200
IP 1:50

Validation Images & Assay Conditions

Click image to see more details

Western blot analysis of ATF5 expression in (1) Jurkat cell lysate; (2) 3T3 cell lysate; (2) C6 cell lysate.

Click image to see more details

All lanes use the Antibody at 1:3K dilution for 1 hour at room temperature.

Click image to see more details

All lanes use the Antibody at 1:3K dilution for 1 hour at room temperature.

Click image to see more details

Immunohistochemical analysis of paraffin-embedded Human breast, using ATF5 Antibody.

Click image to see more details

ICC staining of ATF5 in Hela cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody (1/50) for 1 hour at room temperature, washed with PBS

Click image to see more details

Immunofluorescent analysis using the Antibody at 1:50 dilution.

Click image to see more details

Immunofluorescent analysis using the Antibody at 1:150 dilution.

Gene/Protein Information For ATF5 (Source: Uniprot.org, NCBI)

Alternative Names

activating transcription factor 5FLJ34666; ATFXHMFN0395; cAMP-dependent transcription factor ATF-5; cyclic AMP-dependent transcription factor ATF-5; Transcription factor ATFx ATF5 ATFX, HMFN0395 activating transcription factor 5 cyclic AMP-dependent transcription factor ATF-5|cAMP-dependent transcription factor ATF-5|transcription factor ATFx

For more info on ATF5, check out the ATF5 Infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for ATF5: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Take me to the ATF5 Infographic

Here are featured tools and databases that you might find useful.

• Boster's Pathways Library
• Protein Databases
• Bioscience Research Protocol Resources
• Data Processing & Analysis Software
• Photo Editing Software
• Scientific Literature Resources
• Research Paper Management Tools
• Molecular Biology Software
• Primer Design Tools
• Bioinformatics Tools
• Phylogenetic Tree Analysis