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Product Info Summary
| SKU: | A03546 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, WB |
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Product info
Product Name
Anti-ATG14 Antibody
SKU/Catalog Number
A03546
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-ATG14 Antibody (Catalog # A03546). Tested in ELISA, WB, IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
ATG14 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-ATG14 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03546)
Host
Rabbit
Contents
ATG14 Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
ATG14 antibody was raised against an 18 amino acid synthetic peptide near the center of human ATG14. The immunogen is located within amino acids 270 - 320 of ATG14.
Cross-reactivity
At least three isoforms of ATG14 are known to exist; this antibody will detect all three isoforms
Reactive Species
A03546 is reactive to ATG14 in Human, Mouse, Rat
Observed Molecular Weight
68 kDa
Calculated molecular weight
55.3 kDa
Background of ATG14
Autophagy, the process of bulk degradation of cellular proteins through an autophagosomic-lysosomal pathway is important for normal growth control and may be defective in tumor cells. It is involved in the preservation of cellular nutrients under starvation conditions as well as the normal turnover of cytosolic components. This process is negatively regulated by TOR (Target of rapamycin) through phosphorylation of autophagy protein ATG1. ATG14 is a subunit of a class III phosphatidylinositol (PtdIns) 3-kinase complex that targets the complex to the endoplasmic reticulum, the site of autophagosome formation.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A03546 is guaranteed for ELISA, IF, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
ATG14 antibody can be used for detection of ATG14 by Western blot at 1 - 2 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of ATG14 in human small intestine tissue lysate with ATG14 antibody at (A) 1 and (B) 2 μg/mL.
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Immunofluorescence of ATG14 in human small intestine tissue with ATG14 antibody at 20 μg/mL.
Specific Publications For Anti-ATG14 Antibody (A03546)
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