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Product Info Summary
| SKU: | A00526 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, IF, ICC, WB |
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Product info
Product Name
Anti-ATG16 ATG16L1 Antibody
SKU/Catalog Number
A00526
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-ATG16 ATG16L1 Antibody (Catalog # A00526). Tested in ELISA, WB, ICC, IF applications. This antibody reacts with Human.
Storage & Handling
ATG16 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-ATG16 ATG16L1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00526)
Host
Rabbit
Contents
ATG16 Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
ATG16 antibody was raised against a 18 amino acid synthetic peptide from near the amino terminus of human ATG16. The immunogen is located within amino acids 40 - 90 of ATG16.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
At least two isoforms of KEAP1 are known to exist.
Reactive Species
A00526 is reactive to ATG16L1 in Human
Applications
A00526 is guaranteed for ELISA, IF, ICC, WB Boster Guarantee
Observed Molecular Weight
68 kDa
Calculated molecular weight
68.265kDa
Background of ATG16L1
Autophagy, the process of bulk degradation of cellular proteins through an autophagosomic-lysosomal pathway is important for normal growth control and may be defective in tumor cells. It is involved in the preservation of cellular nutrients under starvation conditions as well as the normal turnover of cytosolic components. This process is negatively regulated by TOR (Target of rapamycin) through phosphorylation of autophagy protein APG1. ATG16, another member of the autophagy protein family, forms a complex with the ATG5-ATG12 conjugate. This multimeric protein has been shown to be essential for autophagosome formation in both yeast and mammals and targets the ATG5-ATG12 complex to the autophagic isolation membrane during the formation of the autophagosome. Because mammalian ATG16 has seven WD-repeats in its C-terminal domain, it has been suggested that these may form a platform for further protein-protein interactions. Multiple isoforms of ATG16 are known to exist.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
ATG16 antibody can be used for the detection of ATG16 by Western blot at 1 and 2 μg/mL. Antibody can also be used for immunocytochemistry starting at 2 μg/mL. For immunofluorescence start at 4 μg/mL.
Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of ATG16 in HeLa cell lysate with ATG16 antibody at (A) 1 and (B) 2 μg/mL.
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Immunocytochemistry of ATG16 in HeLa cells with ATG16 antibody at 2 μg/mL.
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Immunofluorescence of ATG16 in Hela cells with ATG16 antibody at 4.75 μg/mL.
Protein Target Info & Infographic
Gene/Protein Information For ATG16L1 (Source: Uniprot.org, NCBI)
Gene Name
ATG16L1
Full Name
Autophagy-related protein 16-1
Weight
68.265kDa
Superfamily
WD repeat ATG16 family
Alternative Names
APG16 autophagy 16-like (S. cerevisiae); APG16L beta; APG16LFLJ10828; APG16-like 1; ATG16 autophagy related 16-like (S. cerevisiae); ATG16 autophagy related 16-like 1 (S. cerevisiae); ATG16A; ATG16L; autophagy-related protein 16-1; FLJ00045; FLJ10035; FLJ22677; IBD10; WD repeat domain 30; WDR30 ATG16L1 APG16L, ATG16A, ATG16L, IBD10, WDR30 autophagy related 16 like 1 autophagy-related protein 16-1|APG16L beta|ATG16 autophagy related 16-like 1|WD repeat domain 30
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ATG16L1, check out the ATG16L1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ATG16L1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-ATG16 ATG16L1 Antibody (A00526)
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No publications found for A00526
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