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Product Info Summary
| SKU: | A01768 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC-P, WB |
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Product info
Product Name
Anti-ATG3 Antibody
SKU/Catalog Number
A01768
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-ATG3 Antibody (Catalog # A01768). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
ATG3 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-ATG3 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01768)
Host
Rabbit
Contents
ATG3 Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Clone Number
Clone: SK7
Isotype
IgG
Immunogen
ATG3 antibody was raised against an 18 amino acid synthetic peptide near the center of human ATG3. The immunogen is located within amino acids 180 - 230 of ATG3.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
PROM1 antibody is human, mouse and rat reactive. Multiple isoforms of PROM1 are known to exist.
Reactive Species
A01768 is reactive to ATG3 in Human, Mouse, Rat
Applications
A01768 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee
Observed Molecular Weight
68 kDa
Calculated molecular weight
35.864kDa
Background of ATG3/APG3
Autophagy, the process of bulk degradation of cellular proteins through an autophagosomic-lysosomal pathway is important for normal growth control and may be defective in tumor cells. It is involved in the preservation of cellular nutrients under starvation conditions as well as the normal turnover of cytosolic components. This process is negatively regulated by TOR (Target of rapamycin) through phosphorylation of autophagy protein APG1. ATG3 (APG3) is a widely expressed conjugating enzyme for APG8 lipidation, an essential step for the initiation of autophagy. It functions as an E2-like enzyme during the initial stages of autophagosome formation by catalyzing the formation of the Atg8-phosphatidylethanolamine (Atg8-PE) conjugate, which is critical for autophagy.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
ATG3 antibody can be used for detection of ATG3 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in mouse samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of ATG3 in Mouse kidney tissue Lysate with ATG3 antibody at (A) 1 and (B) 2 μg/mL.
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Immunohistochemistry of ATG3 in human kidney tissue with ATG3 antibody at 5 μg/mL.
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Immunofluorescence of ATG3 in human kidney with ATG3 antibody at 20 μg/mL.
Protein Target Info & Infographic
Gene/Protein Information For ATG3 (Source: Uniprot.org, NCBI)
Gene Name
ATG3
Full Name
Ubiquitin-like-conjugating enzyme ATG3
Weight
35.864kDa
Superfamily
ATG3 family
Alternative Names
APG3 autophagy 3-like (S. cerevisiae); APG3; APG3L; APG3-LIKE; Apg3p; ATG3 autophagy related 3 homolog (S. cerevisiae); ATG3; Autophagy-related protein 3; DKFZp564M1178; EC 6.3.2.-; FLJ22125,2610016C12Rik; hApg3; MGC15201; PC3-96; Protein PC3-96; ubiquitin-like-conjugating enzyme ATG3 ATG3 APG3, APG3-LIKE, APG3L, PC3-96 autophagy related 3 ubiquitin-like-conjugating enzyme ATG3|2610016C12Rik|APG3 autophagy 3-like|ATG3 autophagy related 3 homolog|autophagy-related protein 3|hApg3
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ATG3, check out the ATG3 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ATG3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-ATG3 Antibody (A01768)
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No publications found for A01768
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Customer Reviews
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