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- Table of Contents
ATG3 Antibodies
AND ATG3 ELISA kits, ATG3 Proteins
Many biological assays use antibodies to detect ATG3, such as Western Blot (WB), ELISA, Flow Cytometry, Immunocytochemistry (IHC). These antibodies can be polyclonal or monoclonal, and react with ATG3 in Human, Mouse, Rat, and many other animal samples. Boster Bio use mainly mouse and rabbit to develop ATG3 antibodies...
Designing a Western blot for ATG3? The guide has the expected band size, antibodies backed by real blot images, the controls to run alongside, and protocols taken from published papers.
Open the ATG3 Western Blot GuideDesigning an IHC experiment for ATG3? The guide has the expected staining pattern, antigen retrieval and fixation, antibodies backed by real IHC images, the tissue controls to run alongside, and protocols taken from published papers.
Open the ATG3 IHC GuideATG3 Infographic by Boster Bio
All ATG3 Products
Product
Figures
Specificity
Applications
Price
Human
ELISA, Flow Cytometry, IHC, WB
$370
Cat # A01772-2
100 μg/vial
Cat # A01772-2
100 μg/vial
Anti-Apg3 (Atg3) Rabbit Monoclonal Antibody
2 Citations
Human, Mouse, Rat
IF, IHC, ICC, WB
$370
Cat # M01768
100 μl
Cat # M01768
100 μl
Human, Mouse, Rat
ELISA, IP, IF, WB
$146
Cat # M01768-3
20μL, 40μL, 100μL, 200μL
Cat # M01768-3
20μL, 40μL, 100μL, 200μL
ATG3 Overview
Facts about Ubiquitin-like-conjugating enzyme ATG3.
Ubiquitin-like-conjugating enzyme ATG3 (ATG3)
E2 conjugating enzyme required for the cytoplasm to vacuole transport (Cvt), autophagy, and mitochondrial homeostasis. Responsible for the E2-like covalent binding of phosphatidylethanolamine to the C-terminal Gly of ATG8-like proteins (GABARAP, GABARAPL1, GABARAPL2 or MAP1LC3A).
The ATG12- ATG5 conjugate plays a role of an E3 and promotes the transport of ATG8-like proteins from ATG3 to phosphatidylethanolamine (PE). This step is required for the membrane association of ATG8-like proteins.
Gene Information
| Human | |
|---|---|
| Gene Name: | ATG3 |
| Uniprot: | Q9NT62 |
| Entrez: | 64422 |
Family
| Belongs to: |
|---|
| ATG3 family |
Alternative Names
APG3 autophagy 3-like (S. cerevisiae); APG3; APG3L; APG3-LIKE; Apg3p; ATG3 autophagy related 3 homolog (S. cerevisiae); ATG3; Autophagy-related protein 3; DKFZp564M1178; EC 6.3.2.-; FLJ22125,2610016C12Rik; hApg3; MGC15201; PC3-96; Protein PC3-96; ubiquitin-like-conjugating enzyme ATG3
Molecular Weight
Mass (kDA):
35.864 kDA
Genomic Context
| Human | |
|---|---|
| Location: | 3q13.2 |
| Sequence: | 3; NC_000003.12 (112532510..112561962, complement) |
Tissue Specificity
Widely expressed, with a highest expression in heart, skeletal muscle, kidney, liver and placenta.
Subcellular Localizations
Cytoplasm.
Diseases
- Malignant Neoplasms
- Neoplasms
- Leukemia
- Toxoplasmosis
- Refractory Carcinoma
- Carcinoma
- Malaria
- Lymphoma
- Ischemia
- Neurodegenerative Disorders
- Innate Immune Response
- Learning Disorders
Interacting Proteins
- ATG12
- GABARAP
- GABARAPL1
- GABARAPL2
- MAP1LC3B
- TECPR1
Pathways
- Autophagy
- Conjugation
- Cell Death
- Localization
- Macroautophagy
- Cellular Process
- Transport
- Programmed Cell Death
- Immune Response
- Translation
- Catabolic Process
- Rna Interference
- Cvt Pathway
PTMs
- Cleavage
- Phosphorylation
Research Areas
- Autophagy
- Cancer
For details, visit:
bosterbio.com/bosterbio-gene-info-cards/ATG3
bosterbio.com/bosterbio-gene-info-cards/ATG3
References
PMID: 11825910 by Tanida I., et al. Human Apg3p/Aut1p homologue is an authentic E2 enzyme for multiple substrates, GATE-16, GABARAP, and MAP-LC3, and facilitates the conjugation of hApg12p to hApg5p.
PMID: 12207896 by Tanida I., et al. Mammalian Apg12p, but not the Apg12p.Apg5p conjugate, facilitates LC3 processing.