Anti-Atg4C Rabbit Monoclonal Antibody

ATG4C antibody

Boster Bio Anti-Atg4C Rabbit Monoclonal Antibody catalog # M09728. Tested in WB, IHC, Flow Cytometry applications. This antibody reacts with Human.

Product Info Summary

SKU: M09728
Size: 100 μl
Reactive Species: Human
Host: Rabbit
Application: Flow Cytometry, IHC, WB

Product Name

Anti-Atg4C Rabbit Monoclonal Antibody

View all ATG4C Antibodies

SKU/Catalog Number

M09728

Size

100 μl

Form

Liquid

Description

Boster Bio Anti-Atg4C Rabbit Monoclonal Antibody catalog # M09728. Tested in WB, IHC, Flow Cytometry applications. This antibody reacts with Human.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Atg4C Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M09728)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Clonality

Monoclonal

Clone Number

ABIC-1

Isotype

Rabbit IgG

Immunogen

A synthesized peptide derived from human Atg4C

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

M09728 is reactive to ATG4C in Human

Applications

M09728 is guaranteed for Flow Cytometry, IHC, WB Boster Guarantee

Observed Molecular Weight

53 kDa

Calculated molecular weight

52.497kDa

Background of ATG4C

Putative transcription factor involved in pancreas development and function.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB 1:1000-1:5000
IHC 1:50-1:200
FC 1:50

Validation Images & Assay Conditions

Gene/Protein Information For ATG4C (Source: Uniprot.org, NCBI)

Gene Name

ATG4C

Full Name

Cysteine protease ATG4C

Weight

52.497kDa

Superfamily

peptidase C54 family

Alternative Names

APG4 autophagy 4 homolog C (S. cerevisiae); APG4 autophagy 4 homolog C; APG4C; ATG4 autophagy related 4 homolog C (S. cerevisiae); AUT (S. cerevisiae)-like 1, cysteine endopeptidase; AUT-like 1, cysteineendopeptidase (S. cerevisiae); AUTL1; AUTL3APG4-C; AUT-like 1, cysteine endopeptidase; AUT-like 3 cysteine endopeptidase; autophagin-3; Autophagy-related cysteine endopeptidase 3; Autophagy-related protein 4 homolog C; cysteine protease ATG4C; EC 3.4.22; EC 3.4.22.-; FLJ14867 ATG4C APG4-C, APG4C, AUTL1, AUTL3 autophagy related 4C cysteine peptidase cysteine protease ATG4C|APG4 autophagy 4 homolog C|ATG4 autophagy related 4 homolog C|AUT-like 1, cysteine endopeptidase|AUT-like 3 cysteine endopeptidase|autophagin-3|autophagy-related cysteine endopeptidase 3|autophagy-related protein 4 homolog C|epididymis secretory sperm binding protein

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on ATG4C, check out the ATG4C Infographic

ATG4C infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for ATG4C: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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6 Customer Q&As for Anti-Atg4C Rabbit Monoclonal Antibody

Question

I have a question about product M09728, anti-Atg4C Rabbit Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2019-07-03

Answer

We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M09728 anti-Atg4C Rabbit Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2019-07-03

Question

We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for heart using anti-Atg4C Rabbit Monoclonal antibody M09728. Let me know if you need anything else.

Verified Customer

Verified customer

Asked: 2019-06-18

Answer

Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-06-18

Question

Does anti-Atg4C Rabbit Monoclonal antibody M09728 work for WB with heart?

Verified Customer

Verified customer

Asked: 2018-11-29

Answer

According to the expression profile of heart, ATG4C is highly expressed in heart. So, it is likely that anti-Atg4C Rabbit Monoclonal antibody M09728 will work for WB with heart.

Boster Scientific Support

Answered: 2018-11-29

Question

Is this M09728 anti-Atg4C Rabbit Monoclonal antibody reactive to the isotypes of ATG4C?

Verified Customer

Verified customer

Asked: 2018-02-02

Answer

The immunogen of M09728 anti-Atg4C Rabbit Monoclonal antibody is A synthesized peptide derived from human Atg4C. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2018-02-02

Question

We are currently using anti-Atg4C Rabbit Monoclonal antibody M09728 for human tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on canine tissues as well?

L. Evans

Verified customer

Asked: 2016-09-30

Answer

The anti-Atg4C Rabbit Monoclonal antibody (M09728) has not been validated for cross reactivity specifically with canine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2016-09-30

Question

I was wanting to use your anti-Atg4C Rabbit Monoclonal antibody for WB for human heart on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human heart identification?

G. Anderson

Verified customer

Asked: 2013-04-10

Answer

You can see on the product datasheet, M09728 anti-Atg4C Rabbit Monoclonal antibody has been validated for WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human heart in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2013-04-10

Order DetailsPrice
M09728

100μL

$370
M09728-30ul

30μL sample

$99
M09728-carrier-free

Carrier Free

$370

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M09728
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

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