Click image to see more details
Product Info Summary
| SKU: | M09728 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | Flow Cytometry, IHC, WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-Atg4C Rabbit Monoclonal Antibody
SKU/Catalog Number
M09728
BM5175 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-Atg4C Rabbit Monoclonal Antibody catalog # M09728. Tested in WB, IHC, Flow Cytometry applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Atg4C Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M09728)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
ABIC-1
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Atg4C
Reactive Species
M09728 is reactive to ATG4C in Human
Observed Molecular Weight
52 kDa
Calculated molecular weight
52.5 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M09728 is guaranteed for Flow Cytometry, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
IHC 1:50-200
FC 1:20
Positive Control
WB: Jurkat cell
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of Atg4C expression in Jurkat cell lysate.
Specific Publications For Anti-Atg4C Rabbit Monoclonal Antibody (M09728)
Loading publications
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-Atg4C Rabbit Monoclonal Antibody?
Share your experimental results or join a short interview to earn up to $1,000 in product credits or other rewards.
0 Reviews For Anti-Atg4C Rabbit Monoclonal Antibody
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
6 Customer Q&As for Anti-Atg4C Rabbit Monoclonal Antibody
Question
I have a question about product M09728, anti-Atg4C Rabbit Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-07-03
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M09728 anti-Atg4C Rabbit Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-07-03
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for heart using anti-Atg4C Rabbit Monoclonal antibody M09728. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-06-18
Answer
Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-06-18
Question
Does anti-Atg4C Rabbit Monoclonal antibody M09728 work for WB with heart?
Verified Customer
Verified customer
Asked: 2018-11-29
Answer
According to the expression profile of heart, ATG4C is highly expressed in heart. So, it is likely that anti-Atg4C Rabbit Monoclonal antibody M09728 will work for WB with heart.
Boster Scientific Support
Answered: 2018-11-29
Question
Is this M09728 anti-Atg4C Rabbit Monoclonal antibody reactive to the isotypes of ATG4C?
Verified Customer
Verified customer
Asked: 2018-02-02
Answer
The immunogen of M09728 anti-Atg4C Rabbit Monoclonal antibody is A synthesized peptide derived from human Atg4C. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2018-02-02
Question
We are currently using anti-Atg4C Rabbit Monoclonal antibody M09728 for human tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on canine tissues as well?
L. Evans
Verified customer
Asked: 2016-09-30
Answer
The anti-Atg4C Rabbit Monoclonal antibody (M09728) has not been validated for cross reactivity specifically with canine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2016-09-30
Question
I was wanting to use your anti-Atg4C Rabbit Monoclonal antibody for WB for human heart on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human heart identification?
G. Anderson
Verified customer
Asked: 2013-04-10
Answer
You can see on the product datasheet, M09728 anti-Atg4C Rabbit Monoclonal antibody has been validated for WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human heart in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2013-04-10
