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Product Info Summary
| SKU: | A06539-1 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | IF, IHC |
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Product info
Product Name
Anti-Cysteine protease ATG4A ATG4A Antibody
SKU/Catalog Number
A06539-1
Size
100 µl
Form
Liquid
Description
Boster Bio Anti-Cysteine protease ATG4A ATG4A Antibody (Catalog # A06539-1). Tested in IHC, IF applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Cysteine protease ATG4A ATG4A Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A06539-1)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from C-terminal of human ATG4A.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A06539-1 is reactive to ATG4A in Human, Mouse
Applications
A06539-1 is guaranteed for IF, IHC Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
45.378kDa
Background of ATG4A
Cysteine protease required for the cytoplasm to vacuole transport (Cvt) and autophagy. Cleaves the C-terminal amino acid of ATG8 family proteins to reveal a C-terminal glycine. Exposure of the glycine at the C-terminus is essential for ATG8 proteins conjugation to phosphatidylethanolamine (PE) and insertion to membranes, which is necessary for autophagy. Preferred substrate is GABARAPL2 followed by MAP1LC3A and GABARAP. Has also an activity of delipidating enzyme for the PE-conjugated forms.
Marino G., J. Biol. Chem. 278:3671-3678(2003).
Kabeya Y., J. Cell Sci. 117:2805-2812(2004).
Ross M.T., Nature 434:325-337(2005
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Reconsitution
Restore with deionized water (or equivalent) for reconstitution volume of 100 µL
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry: 1:50~1:100
Immunofluorescence: 1:100~1:500
Validation Images & Assay Conditions
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Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using ATG4A antibody A06539-1.
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Immunofluorescence analysis of A549 cells, using ATG4A antibody A06539-1.
Protein Target Info & Infographic
Gene/Protein Information For ATG4A (Source: Uniprot.org, NCBI)
Gene Name
ATG4A
Full Name
Cysteine protease ATG4A
Weight
45.378kDa
Superfamily
peptidase C54 family
Alternative Names
APG4 autophagy 4 homolog A (S. cerevisiae); Apg4a; ATG4 autophagy related 4 homolog A (S. cerevisiae); ATG4A; Atg4al; Autl2; AUT-like 2 cysteine endopeptidase; AUT-like 2, cysteine endopeptidase (S. cerevisiae); Autophagin 2; autophagin-2; cysteine endopeptidase; cysteine protease ATG4A; EC 3.4.22; EC 3.4.22.-; hAPG4A ATG4A APG4A, AUTL2 autophagy related 4A cysteine peptidase cysteine protease ATG4A|APG4 autophagy 4 homolog A|ATG4 autophagy related 4 homolog A|AUT-like 2, cysteine endopeptidase|autophagin 2|autophagy-related cysteine endopeptidase 2|autophagy-related protein 4 homolog A|hAPG4A
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ATG4A, check out the ATG4A Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ATG4A: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Cysteine protease ATG4A ATG4A Antibody (A06539-1)
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No publications found for A06539-1
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