Anti-Atg4B Rabbit Monoclonal Antibody

Product Name

Anti-Atg4B Rabbit Monoclonal Antibody

View all ATG4B Antibodies

SKU/Catalog Number

M02885

Size

100 μl

Form

Liquid

Description

Boster Bio Anti-Atg4B Rabbit Monoclonal Antibody catalog # M02885. Tested in WB, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Atg4B Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M02885)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Clonality

Monoclonal

Clone Number

ABIA-1

Isotype

Rabbit IgG

Immunogen

A synthesized peptide derived from human Atg4B

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

M02885 is reactive to ATG4B in Human, Mouse, Rat

Applications

M02885 is guaranteed for Flow Cytometry, WB Boster Guarantee

Observed Molecular Weight

50 kDa

Calculated molecular weight

44.294kDa

Background of ATG4B

F-actin cross-linking protein which is thought to anchor actin to a variety of intracellular structures. This is a bundling protein. Probably involved in vesicular trafficking via its association with the CART complex. The CART complex is necessary for efficient transferrin receptor recycling but not for EGFR degradation.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB 1:500-1:2000
FC 1:100

Validation Images & Assay Conditions

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All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.

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Western blot analysis of Atg4B expression in Ramos cell lysate.

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All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.

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Immunofluorescent analysis using the Antibody at 1:50 dilution.

Gene/Protein Information For ATG4B (Source: Uniprot.org, NCBI)

Alternative Names

APG4 autophagy 4 homolog B (S. cerevisiae); APG4 autophagy 4 homolog B; Apg4B; ATG4 autophagy related 4 homolog B (S. cerevisiae); ATG4B; AUTL1; AUTL1MGC1353; AUT-like 1 cysteine endopeptidase; autophagin-1; Autophagy-related cysteine endopeptidase 1; Autophagy-related protein 4 homolog B; cysteine protease ATG4B; DKFZp586D1822; EC 3.4.22; EC 3.4.22.-; hAPG4B; KIAA0943 ATG4B APG4B, AUTL1 autophagy related 4B cysteine peptidase cysteine protease ATG4B|APG4 autophagy 4 homolog B|ATG4 autophagy related 4 homolog B|AUT-like 1 cysteine endopeptidase|autophagin-1|autophagy-related cysteine endopeptidase 1|autophagy-related protein 4 homolog B|hAPG4B

For more info on ATG4B, check out the ATG4B Infographic

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In this infographic, you will see the following information for ATG4B: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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