Anti-ATM Monoclonal Antibody

Product Name

Anti-ATM Monoclonal Antibody

View all ATM Antibodies

SKU/Catalog Number

M00014

Size

100 μl

Form

Liquid

Description

Boster Bio Anti-ATM Monoclonal Antibody catalog # M00014. Tested in WB, IHC, ICC/IF, Flow Cytometry applications. This antibody reacts with Human.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-ATM Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00014)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Clonality

Monoclonal

Clone Number

BCF-1

Isotype

Rabbit IgG

Immunogen

A synthesized peptide derived from human ATM

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

M00014 is reactive to ATM in Human

Applications

M00014 is guaranteed for Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee

Observed Molecular Weight

351 kDa

Calculated molecular weight

350.687kDa

Background of ATM

The ion channels activated by glutamate are typically divided into two classes. Those that are sensitive to N-methyl-D-aspartate (NMDA) are designated NMDA receptors (NMDAR) while those activated by α-amino-3-hydroxy-5-methyl-4-isoxalone propionic acid (AMPA) are known as AMPA receptors (AMPAR). The AMPAR are comprised of four distinct glutamate receptor subunits designated (GluR1-4) and they play key roles in virtually all excitatory neurotransmission in the brain (Keinänen et al., 1990; Hollmann and Heinemann, 1994). The GluR1 subunit is widely expressed throughout the nervous system. Phosphorylation of Ser-845 on GluR1 is thought to be mediated by PKA and phosphorylation of this site increases the conductance of the AMPAR (Roche et al., 1996; Banke et al., 2000). In addition, phosphorylation of this site has been linked to synaptic plasticity as well as learning and memory (Lee at al., 2003; Esteban at al., 2003).

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB 1:500-1:2000
IHC 1:50-1:100
ICC/IF 1:50-1:200
FC 1:50

Validation Images & Assay Conditions

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Figure 1. Western blot analysis of ATM using anti-ATM antibody (M00014).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human HepG2 whole cell lysates,
Lane 2: human 293T whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ATM antigen affinity purified polyclonal antibody (Catalog # M00014) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ATM at approximately 351 kDa. The expected band size for ATM is at 351 kDa.

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Immunohistochemical analysis of paraffin-embedded human breast cancer, using ATM Antibody.

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Immunofluorescent analysis of Hela cells, using ATM Antibody.

Gene/Protein Information For ATM (Source: Uniprot.org, NCBI)

Alternative Names

AT mutated; A-T mutated; AT1; ATA; ataxia telangiectasia mutated (includes complementation groups A, C and D); ataxia telangiectasia mutatedATD; ATC; ATDC; ATE; ATM; DKFZp781A0353; EC 2.7.11.1; MGC74674; serine-protein kinase ATM; TEL1; TEL1, telomere maintenance 1, homolog; TELO1; TPLL ATM AT1, ATA, ATC, ATD, ATDC, ATE, TEL1, TELO1 ATM serine/threonine kinase serine-protein kinase ATM|A-T mutated|AT mutated|TEL1, telomere maintenance 1, homolog|ataxia telangiectasia mutated

For more info on ATM, check out the ATM Infographic

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In this infographic, you will see the following information for ATM: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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