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Product Info Summary
| SKU: | A04657 |
|---|---|
| Size: | 80 µl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | Flow Cytometry, IHC-P, WB |
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Product info
Product Name
Anti-ATP2A1 Antibody (C-term)
View all SERCA1 ATPase Antibodies
SKU/Catalog Number
A04657
Size
80 µl
Description
Boster Bio Anti-ATP2A1 Antibody (C-term) (Catalog # A04657). Tested in WB, Flow Cytometry, IHC-P application(s). This antibody reacts with Human.
Storage & Handling
Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Cite This Product
Anti-ATP2A1 Antibody (C-term) (Boster Biological Technology, Pleasanton CA, USA, Catalog # A04657)
Host
Rabbit
Contents
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
This ATP2A1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 974-1001 amino acids from the C-terminal region of human ATP2A1.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A04657 is reactive to ATP2A1 in Human
Applications
A04657 is guaranteed for Flow Cytometry, IHC-P, WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
110.252kDa
Background of SERCA1 ATPase
ATP2A1 encodes one of the SERCA Ca(2+)-ATPases, which are intracellular pumps located in the sarcoplasmic or endoplasmic reticula of muscle cells. This enzyme catalyzes the hydrolysis of ATP coupled with the translocation of calcium from the cytosol to the sarcoplasmic reticulum lumen, and is involved in muscular excitation and contraction.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:1000
IHC-P: 1:50-1:100
FC: 1:10-1:50
Validation Images & Assay Conditions
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Western blot analysis of lysates from RD,HT1080 cell line (from left to right),using ATP2A1 Antibody (C-term).A04657 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:5000 dilution was used as the secondary antibody.Lysates at 35ug per lane.
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ATP2A1 Antibody (C-term) IHC analysis in formalin fixed and paraffin embedded human skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the ATP2A1 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
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ATP2A1 Antibody (C-term) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Protein Target Info & Infographic
Gene/Protein Information For ATP2A1 (Source: Uniprot.org, NCBI)
Gene Name
ATP2A1
Full Name
Sarcoplasmic/endoplasmic reticulum calcium ATPase 1
Weight
110.252kDa
Superfamily
cation transport ATPase (P-type) (TC 3.A.3) family
Alternative Names
ATP2A; ATPase, Ca++ transporting, cardiac muscle, fast twitch 1; Calcium pump 1; Calcium-transporting ATPase sarcoplasmic reticulum type, fast twitch skeletalmuscle isoform; EC 3.6.3; Endoplasmic reticulum class 1/2 Ca(2+) ATPase; sarcoplasmic/endoplasmic reticulum calcium ATPase 1; SERCA1EC 3.6.3.8; SR Ca(2+)-ATPase 1 ATP2A1 ATP2A, SERCA1 ATPase sarcoplasmic/endoplasmic reticulum Ca2+ transporting 1 sarcoplasmic/endoplasmic reticulum calcium ATPase 1|ATPase, Ca++ transporting, cardiac muscle, fast twitch 1|SR Ca(2+)-ATPase 1|calcium pump 1|endoplasmic reticulum class 1/2 Ca(2+) ATPase
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ATP2A1, check out the ATP2A1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ATP2A1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-ATP2A1 Antibody (C-term) (A04657)
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No publications found for A04657
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