Product Info Summary
SKU: | A09735 |
---|---|
Size: | 100 μg/vial |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | ELISA, Flow Cytometry, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-ATP5G1,2,3/ATP5MC1,2,3 Antibody Picoband™
View all ATP synthase C mature Antibodies
SKU/Catalog Number
A09735
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-ATP5G1,2,3/ATP5MC1,2,3 Antibody Picoband™ catalog # A09735. Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-ATP5G1,2,3/ATP5MC1,2,3 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A09735)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human ATP5F1/ATP5MC1 recombinant protein (Position: D62-L113).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A09735 is reactive to ATP5MC1 in Human, Mouse, Rat
Applications
A09735 is guaranteed for ELISA, Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee
Observed Molecular Weight
10-14 kDa
Calculated molecular weight
14.277kDa
Background of ATP synthase C mature
The ATP5MC1 gene is one of three human paralogs that encode membrane subunit c of the mitochondrial ATP synthase. It is mapped to 17q21.32. This gene encodes a subunit of mitochondrial ATP synthase. Mitochondrial ATP synthase catalyzes ATP synthesis, utilizing an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. ATP synthase is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, comprising the proton channel. The catalytic portion of mitochondrial ATP synthase consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled with a stoichiometry of 3 alpha, 3 beta, and a single representative of the other 3. The proton channel seems to have nine subunits (a, b, c, d, e, f, g, F6 and 8). This gene is one of three genes that encode subunit c of the proton channel. Each of the three genes have distinct mitochondrial import sequences but encode the identical mature protein. Alternatively spliced transcript variants encoding the same protein have been identified.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Reconsitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat<, By Heat
Immunofluorescence, 2μg/ml, Human
Immunocytochemistry/Immunofluorescence, 2μg/ml, Human
Flow Cytometry, 1-3μg/1x106 cells, Human
Direct ELISA, 0.1-0.5μg/ml, Human
Validation Images & Assay Conditions
Click image to see more details
Figure 1. IF analysis of ATP5G1,2,3 using anti-ATP5G1,2,3 antibody (A09735).
ATP5G1,2,3 was detected in paraffin-embedded section of human intestinal tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/mL rabbit anti-ATP5G1,2,3 Antibody (A09735) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG (BA1127) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
Click image to see more details
Figure 10. IHC analysis of ATP5G1,2,3 using anti-ATP5G1,2,3 antibody (A09735). ATP5G1,2,3 was detected in paraffin-embedded section of rat intestine tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-ATP5G1,2,3 Antibody (A09735) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 2. Flow Cytometry analysis of HL-60 cells using anti-ATP5G1,2,3 antibody (A09735).
Overlay histogram showing HL-60 cells stained with A09735 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ATP5G1,2,3 Antibody (A09735,1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Click image to see more details
Figure 3. Flow Cytometry analysis of PC-3 cells using anti-ATP5G1,2,3 antibody (A09735).
Overlay histogram showing HL-60 cells stained with A09735 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ATP5G1,2,3 Antibody (A09735,1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Click image to see more details
Figure 4. IHC analysis of ATP5G1,2,3 using anti-ATP5G1,2,3 antibody (A09735).
ATP5G1,2,3 was detected in paraffin-embedded section of human Ovarian cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-ATP5G1,2,3 Antibody (A09735) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 5. IHC analysis of ATP5G1,2,3 using anti-ATP5G1,2,3 antibody (A09735).
ATP5G1,2,3 was detected in paraffin-embedded section of human tonsil tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-ATP5G1,2,3 Antibody (A09735) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 6. IHC analysis of ATP5G1,2,3 using anti-ATP5G1,2,3 antibody (A09735).
ATP5G1,2,3 was detected in paraffin-embedded section of human glioma tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-ATP5G1,2,3 Antibody (A09735) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 7. IHC analysis of ATP5G1,2,3 using anti-ATP5G1,2,3 antibody (A09735).
ATP5G1,2,3 was detected in paraffin-embedded section of human lung cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-ATP5G1,2,3 Antibody (A09735) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 8. IHC analysis of ATP5G1,2,3 using anti-ATP5G1,2,3 antibody (A09735).
ATP5G1,2,3 was detected in paraffin-embedded section of human placenta tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-ATP5G1,2,3 Antibody (A09735) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 9. IHC analysis of ATP5G1,2,3 using anti-ATP5G1,2,3 antibody (A09735).
ATP5G1,2,3 was detected in paraffin-embedded section of mouse intestine tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-ATP5G1,2,3 Antibody (A09735) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 11. Western blot analysis of ATP5G1,2,3 using anti-ATP5G1,2,3 antibody (A09735).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human HEPG2 whole cell lysates,
Lane 2: human A549 whole cell lysates,
Lane 3: human PC-3 whole cell lysates,
Lane 4: human HL-60 whole cell lysates,
Lane 5: human HEK293 whole cell lysates,
Lane 6: human U937 whole cell lysates,
Lane 7: human CACO-2 whole cell lysates,
Lane 8 :rat kidney tissue lysates,
Lane 9: rat stomach tissue lysates,
Lane 10: rat liver tissue lysates,
Lane 11: mouse kidney tissue lysates,
Lane 12: mouse stomach tissue lysates,
Lane 13: mouse liver tissue lysates,
Lane 14: mouse lung tissue lysates,
Lane 15: mouse Neuro-2a whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ATP5G1,2,3 antigen affinity purified polyclonal antibody (Catalog # A09735) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ATP5G1,2,3 at approximately 10-14KD. The expected band size for ATP5G1,2,3 is at 10-14KD.
Protein Target Info & Infographic
Gene/Protein Information For ATP5MC1 (Source: Uniprot.org, NCBI)
Gene Name
ATP5MC1
Full Name
ATP synthase F(0) complex subunit C1, mitochondrial
Weight
14.277kDa
Superfamily
ATPase C chain family
Alternative Names
ATP synthase, H+ transporting, mitochondrial F0 complex, subunit c (subunit 9); ATP synthase, H+ transporting, mitochondrial Fo complex, subunit C1 (subunit 9); ATP5A; ATP5G; ATPase protein 9; ATPase subunit 9; H+ transporting, mitochondrial F0 complex, subunit C1 (subunit 9); isoform 1; mitochondrial ATP synthase, subunit 9; mitochondrial ATP synthase, subunit C; mitochondrial ATP5MC1 ATP5A, ATP5G, ATP5G1 ATP synthase membrane subunit c locus 1 ATP synthase F(0) complex subunit C1, mitochondrial|ATP synthase lipid-binding protein, mitochondrial|ATP synthase proteolipid P1|ATP synthase proton-transporting mitochondrial F(0) complex subunit C1|ATP synthase subunit 9|ATP synthase, H+ transporting, mitochondrial F0 complex, subunit C1 (subunit 9)|ATP synthase, H+ transporting, mitochondrial F0 complex, subunit c (subunit 9)|ATP synthase, H+ transporting, mitochondrial Fo complex subunit C1 (subunit 9)|ATPase protein 9|ATPase subunit 9|ATPase subunit C|dicyclohexylcarbodiimide (DCCD)-reactive proteolipid subunit|mitochondrial ATP synthase, subunit 9|mitochondrial ATP synthase, subunit C
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ATP5MC1, check out the ATP5MC1 Infographic
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In this infographic, you will see the following information for ATP5MC1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-ATP5G1,2,3/ATP5MC1,2,3 Antibody Picoband™ (A09735)
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