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Product Info Summary
| SKU: | A32270-1 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse, Rat, Zebrafish |
| Host: | Rabbit |
| Application: | IP, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-ATPB ATP5F1B Antibody
SKU/Catalog Number
A32270-1
Size
100ul
Form
Liquid
Description
Boster Bio Anti-ATPB ATP5F1B Antibody catalog # A32270-1. Tested in WB,IP,ICC/IF,IHC applications. This antibody reacts with Human,Mouse,Rat,Zebrafish.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-ATPB ATP5F1B Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A32270-1)
Host
Rabbit
Contents
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Clonality
Polyclonal
Isotype
IgG
Immunogen
Recombinant full length Human ATP5B.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A32270-1 is reactive to ATP5F1B in Human, Mouse, Rat, Zebrafish
Applications
A32270-1 is guaranteed for IP, IF, IHC, ICC, WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
56.56kDa
Background of ATPB
Catalytic subunit of a TRAMP-like complex which has a poly (A) RNA polymerase activity and is involved in a post-transcriptional quality control mechanism. Polyadenylation with short oligo (A) tails is required for the degradative activity of the exosome on several of its nuclear RNA substrates. Has no terminal uridylyltransferase activity, and does not play a role in replication-dependent histone mRNA degradation via uridylation.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:1,000-1:5,000
ICC: 1:100-1:500
IHC: 1:50-1:200
IP: 1:10-1:50
Validation Images & Assay Conditions
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Figure 2. Immunocytochemistry staining of ATP5F1B using Anti-ATPB ATP5F1B Antibody (A32270-1).
ICC staining ATPB in 293T cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde
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Figure 1. Western blotting validation for Anti-ATPB ATP5F1B Antibody A32270-1
Western blot analysis of ATPB on different cells lysates using anti-ATPB antibody at 1/500 dilution. Positive control: Line1: Hela Line2: HepG2 Line3: 293TElectrophoresis was performed on a SDS-PAGE gel. To determine SDS-PAGE gel concentration
Protein Target Info & Infographic
Gene/Protein Information For ATP5F1B (Source: Uniprot.org, NCBI)
Gene Name
ATP5F1B
Full Name
ATP synthase subunit beta, mitochondrial
Weight
56.56kDa
Superfamily
ATPase alpha/beta chains family
Alternative Names
ATP synthase subunit beta, mitochondrial; ATP synthase, H+ transporting, mitochondrial F1 complex, beta polypeptide; ATPMB; ATPSBmitochondrial ATP synthase beta subunit; EC 3.6.3; EC 3.6.3.14; MGC5231; mitochondrial ATP synthetase, beta subunit ATP5F1B ATP5B, ATPMB, ATPSB, HEL-S-271 ATP synthase F1 subunit beta ATP synthase subunit beta, mitochondrial|ATP synthase, H+ transporting, mitochondrial F1 complex, beta polypeptide|epididymis secretory protein Li 271|mitochondrial ATP synthase beta subunit|mitochondrial ATP synthetase, beta subunit
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ATP5F1B, check out the ATP5F1B Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ATP5F1B: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-ATPB ATP5F1B Antibody (A32270-1)
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No publications found for A32270-1
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Customer Reviews
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