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Product Info Summary
| SKU: | A03757 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC-P, WB |
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Product info
Product Name
Anti-Autophagy-related protein 9A ATG9A Antibody
SKU/Catalog Number
A03757
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-Autophagy-related protein 9A ATG9A Antibody (Catalog # A03757). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
ATG9A antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-Autophagy-related protein 9A ATG9A Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03757)
Host
Rabbit
Contents
ATG9A Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Clone Number
Clone: SK7
Isotype
IgG
Immunogen
ATG9A antibody was raised against an 18 amino acid synthetic peptide near the carboxy terminus of human ATG9A. The immunogen is located within amino acids 720 - 770 of ATG9A.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
WAPL antibody is human, mouse and rat reactive. At least four isoforms of WAPL are known to exist.
Reactive Species
A03757 is reactive to ATG9A in Human, Mouse, Rat
Applications
A03757 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee
Observed Molecular Weight
68 kDa
Calculated molecular weight
94.447kDa
Background of ATG9A
Autophagy, the process of bulk degradation of cellular proteins through an autophagosomic-lysosomal pathway is important for normal growth control and may be defective in tumor cells. It is involved in the preservation of cellular nutrients under starvation conditions as well as the normal turnover of cytosolic components. This process is negatively regulated by TOR (Target of rapamycin) through phosphorylation of autophagy protein APG1. ATG9A, a multi-spanning membrane protein localizing to the Golgi apparatus and late endosomes, has been proposed to mediate membrane transport to generate autophagosomes. ATG9A has also been implicated as a regulator of STING (stimulator of interferon genes)-mediated innate immune response.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
ATG9A antibody can be used for detection of ATG9A by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in mouse samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of ATG9A in mouse heart tissue lysate with ATG9A antibody at 1 μg/mL.
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Immunohistochemistry of ATG9A in human heart tissue with ATG9A antibody at 5 μg/mL.
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Immunofluorescence of ATG9A in human heart tissue with ATG9A antibody at 20 μg/mL.
Protein Target Info & Infographic
Gene/Protein Information For ATG9A (Source: Uniprot.org, NCBI)
Gene Name
ATG9A
Full Name
Autophagy-related protein 9A
Weight
94.447kDa
Superfamily
ATG9 family
Alternative Names
APG9 autophagy 9-like 1 (S. cerevisiae); APG9 autophagy 9-like 1; APG9L1APG9-like 1; ATG9 autophagy related 9 homolog A (S. cerevisiae); autophagy 9-like 1 protein; autophagy-related protein 9A; FLJ22169; mATG9; MGD3208 ATG9A APG9L1, MGD3208, mATG9 autophagy related 9A autophagy-related protein 9A|APG9 autophagy 9-like 1|APG9-like 1|ATG9 autophagy related 9 homolog A|autophagy 9-like 1 protein
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ATG9A, check out the ATG9A Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ATG9A: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Autophagy-related protein 9A ATG9A Antibody (A03757)
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No publications found for A03757
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