Product Info Summary
SKU: | A03279 |
---|---|
Size: | 100 μg/vial |
Reactive Species: | Human |
Host: | Rabbit |
Application: | ELISA, WB |
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Product info
Product Name
Anti-AVPR1A/V1aR Antibody Picoband™
View all V1a Vasopressin R/AVPR1A Antibodies
SKU/Catalog Number
A03279
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-AVPR1A/V1aR Antibody Picoband™ catalog # A03279. Tested in ELISA, WB applications. This antibody reacts with Human.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-AVPR1A/V1aR Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03279)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human AVPR1A/V1aR recombinant protein (Position: R43-K268).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A03279 is reactive to AVPR1A in Human
Applications
A03279 is guaranteed for ELISA, WB Boster Guarantee
Observed Molecular Weight
60 kDa
Calculated molecular weight
46.8kDa
Background of V1a Vasopressin R/AVPR1A
Arginine vasopressin receptor 1A (officially called AVPR1A) is one of the three major receptor types for arginine vasopressin (AVPR1B and AVPR2 being the others). It belongs to the subfamily of G-protein coupled receptors which includes AVPR1B, V2R and OXT receptors. This gene is mapped to 12q14.2. AVPR1A is present throughout the brain, as well as in the periphery, in the liver, kidney, and vasculature. The protein encoded by this gene acts as receptor for arginine vasopressin. Its activity is mediated by G proteins which stimulate a phosphatidylinositol-calcium second messenger system. The receptor mediates cell contraction and proliferation, platelet aggregation, release of coagulation factor and glycogenolysis.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Reconsitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5μg/ml, Human
Direct ELISA, 0.1-0.5μg/ml, Human
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of AVPR1A using anti-AVPR1A antibody (A03279).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human A549 whole cell lysates,
Lane 2: human A431 whole cell lysates,
Lane 3: human HEK293 whole cell lysates,
Lane 4: human THP-1 whole cell lysates,
Lane 5: human Caco-2 whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-AVPR1A antigen affinity purified polyclonal antibody (Catalog # A03279) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for AVPR1A at approximately 60KD. The expected band size for AVPR1A is at 47KD.
Protein Target Info & Infographic
Gene/Protein Information For AVPR1A (Source: Uniprot.org, NCBI)
Gene Name
AVPR1A
Full Name
Vasopressin V1a receptor
Weight
46.8kDa
Superfamily
G-protein coupled receptor 1 family
Alternative Names
Antidiuretic hormone receptor 1a; arginine vasopressin receptor 1A; AVPR V1a; AVPR1; AVPR1A; SCCL vasopressin subtype 1a receptor; V1a Vasopressin R; V1a vasopressin receptor; V1a VasopressinR; V1aR; V1-vascular vasopressin receptor AVPR1A; Vascular/hepatic-type arginine vasopressin receptor; vasopressin V1a receptor AVPR1A AVPR V1a, AVPR1, V1aR arginine vasopressin receptor 1A vasopressin V1a receptor|SCCL vasopressin subtype 1a receptor|V1-vascular vasopressin receptor AVPR1A|V1a vasopressin receptor|antidiuretic hormone receptor 1A|vascular/hepatic-type arginine vasopressin receptor
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on AVPR1A, check out the AVPR1A Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for AVPR1A: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-AVPR1A/V1aR Antibody Picoband™ (A03279)
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No publications found for A03279
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