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Product Info Summary
| SKU: | M01772 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IF, IHC, ICC, WB |
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Product info
Product Name
Anti-Axin2/Conductin Rabbit Monoclonal Antibody
SKU/Catalog Number
M01772
BM5071 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-Axin2/Conductin Rabbit Monoclonal Antibody catalog # M01772. Tested in WB, IHC, ICC/IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Axin2/Conductin Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01772)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
AAIA-1
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Axin2
Reactive Species
M01772 is reactive to AXIN2 in Human, Mouse, Rat
Observed Molecular Weight
94 kDa
Calculated molecular weight
93.6 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M01772 is guaranteed for IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:50-1:200
ICC/IF 1:50-1:200
Positive Control
WB: SW480 cell, PC-12 cell
Validation Images & Assay Conditions
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Western blot analysis of Axin2 expression in (1) SW480 cell lysate; (2) PC-12 cell lysate.
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Ax increases the expression of GCase and β-catenin when NSCs exposed to OGD. (A–C) Statistical analysis of GCase (A) , β-catenin (B) and Axin2 (C) mRNA expression using RT-qPCR assays in different groups. * P < 0.05, ** P < 0.01 vs. control group; # P < 0.05 vs. OGD + Vehicle; @ P < 0.05 vs. OGD + Ax-30 μM. (D) Bands depicted the expression of GCase, β-catenin, p-β-catenin and Axin2 in different groups. GAPDH was used as an internal control. (E–H) Semi-quantitative analysis of β-catenin (E) , p-β-catenin (F) , GCase (G) and Axin2 (H) expression from (D) . * P < 0.05, ** P < 0.01 vs. control group; # P < 0.05 vs. OGD + Vehicle; @ P < 0.05 vs. OGD + Ax-30 μM. (I) Representative immunostaining images of GCase (green), β-catenin (red) and Nestin (white) in various groups. Scale bar: 20 μm. Ax, ambroxol; OGD, oxygen glucose deprivation.
Index in PubMed under a CC BY license. PMID: 33551744
Specific Publications For Anti-Axin2/Conductin Rabbit Monoclonal Antibody (M01772)
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5 Customer Q&As for Anti-Axin2/Conductin Rabbit Monoclonal Antibody
Question
I was wanting to use your anti-Axin2/Conductin Rabbit Monoclonal antibody for IF for human brain on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human brain identification?
Verified Customer
Verified customer
Asked: 2020-04-06
Answer
As indicated on the product datasheet, M01772 anti-Axin2/Conductin Rabbit Monoclonal antibody has been validated for IF, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in human brain in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-04-06
Question
Will anti-Axin2/Conductin Rabbit Monoclonal antibody M01772 work for IF with brain?
Verified Customer
Verified customer
Asked: 2020-03-25
Answer
According to the expression profile of brain, AXIN2 is highly expressed in brain. So, it is likely that anti-Axin2/Conductin Rabbit Monoclonal antibody M01772 will work for IF with brain.
Boster Scientific Support
Answered: 2020-03-25
Question
Does M01772 anti-Axin2/Conductin Rabbit Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2020-02-17
Answer
You can see on the product datasheet, M01772 anti-Axin2/Conductin Rabbit Monoclonal antibody as been tested on IF. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2020-02-17
Question
Can you help my question with product M01772, anti-Axin2/Conductin Rabbit Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2018-07-05
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M01772 anti-Axin2/Conductin Rabbit Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2018-07-05
Question
We are currently using anti-Axin2/Conductin Rabbit Monoclonal antibody M01772 for human tissue, and we are satisfied with the IF results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on zebrafish tissues as well?
Verified Customer
Verified customer
Asked: 2018-01-31
Answer
The anti-Axin2/Conductin Rabbit Monoclonal antibody (M01772) has not been tested for cross reactivity specifically with zebrafish tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-01-31
