Anti-AZI2 Antibody

Azi2 antibody

Boster Bio Anti-AZI2 Antibody (Catalog # A08235). Tested in WB applications. This antibody reacts with Human, Mouse, Rat.

Product Info Summary

SKU: A08235
Size: 100 µl
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: WB

Product Name

Anti-AZI2 Antibody

View all Azi2 Antibodies

SKU/Catalog Number

A08235

Size

100 µl

Form

Liquid

Description

Boster Bio Anti-AZI2 Antibody (Catalog # A08235). Tested in WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-AZI2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A08235)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Clonality

Polyclonal

Isotype

IgG

Immunogen

Synthesized peptide derived from N-terminal of human AZI2.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A08235 is reactive to Azi2 in Human, Mouse, Rat

Applications

A08235 is guaranteed for WB Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

46.091kDa

Background of Azi2

Adapter protein which binds TBK1 and IKBKE playing a role in antiviral innate immunity. Activates serine/threonine-protein kinase TBK1 and facilitates its oligomerization. Enhances the phosphorylation of NF-kappa-B p65 subunit RELA by TBK1. Promotes TBK1-induced as well as TNF-alpha or PMA-induced activation of NF-kappa-B. Participates in IFNB promoter activation via TICAM1.

Fujita F., Mol. Cell. Biol. 23:7780-7793(2003).
Ota T., Nat. Genet. 36:40-45(2004).
Sasai M., J. Immunol. 174:27-30(2005).

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blotting: 1:500~1:3000

Validation Images & Assay Conditions

Gene/Protein Information For Azi2 (Source: Uniprot.org, NCBI)

Gene Name

Azi2

Full Name

5-azacytidine-induced protein 2

Weight

46.091kDa

Alternative Names

5-azacytidine induced 2; AZ25-azacytidine-induced protein 2; FLJ21939; NAP15-azacytidine induced gene 2; NF-kappa-B-activating kinase-associated protein 1; TILPNak-associated protein 1 Azi2|AA410145, AZ, AZ2|5-azacytidine induced gene 2|5-azacytidine-induced protein 2|NF-kappa-B-activating kinase-associated protein 1|nak-associated protein 1

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on Azi2, check out the Azi2 Infographic

Azi2 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for Azi2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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6 Customer Q&As for Anti-AZI2 Antibody

Question

Does A08235 anti-AZI2 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2019-12-27

Answer

It shows on the product datasheet, A08235 anti-AZI2 antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2019-12-27

Question

I have attached the WB image, lot number and protocol we used for embryo hepatoma using anti-AZI2 antibody A08235. Please let me know if you require anything else.

Verified Customer

Verified customer

Asked: 2019-10-01

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-10-01

Question

I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for embryo hepatoma using anti-AZI2 antibody A08235. Let me know if you need anything else.

Verified Customer

Verified customer

Asked: 2017-09-13

Answer

We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2017-09-13

Question

We are currently using anti-AZI2 antibody A08235 for human tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on primate tissues as well?

J. Singh

Verified customer

Asked: 2016-08-09

Answer

The anti-AZI2 antibody (A08235) has not been tested for cross reactivity specifically with primate tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2016-08-09

Question

Will anti-AZI2 antibody A08235 work for WB with embryo hepatoma?

G. Thomas

Verified customer

Asked: 2016-03-03

Answer

According to the expression profile of embryo hepatoma, AZI2 is highly expressed in embryo hepatoma. So, it is likely that anti-AZI2 antibody A08235 will work for WB with embryo hepatoma.

Boster Scientific Support

Answered: 2016-03-03

Question

I was wanting to use your anti-AZI2 antibody for WB for human embryo hepatoma on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human embryo hepatoma identification?

K. Kulkarni

Verified customer

Asked: 2014-11-14

Answer

You can see on the product datasheet, A08235 anti-AZI2 antibody has been validated for WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in human embryo hepatoma in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2014-11-14

Order DetailsPrice
A08235-100uL

100uL

$360

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A08235
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*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
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