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Product Info Summary
| SKU: | A00456-6 |
|---|---|
| Size: | 80 µl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | Flow Cytometry, IHC-P, WB |
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Product info
Product Name
Anti-B2M Antibody (C-term)
SKU/Catalog Number
A00456-6
Size
80 µl
Form
Liquid
Description
Boster Bio Anti-B2M Antibody (C-term) (Catalog # A00456-6). Tested in WB, IHC-P, Flow Cytometry application(s). This antibody reacts with Human.
Storage & Handling
Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Cite This Product
Anti-B2M Antibody (C-term) (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00456-6)
Host
Rabbit
Contents
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
This B2M antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 86-118 amino acids from the C-terminal region of human B2M.
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A00456-6 is reactive to B2M in Human
Calculated molecular weight
13.7 kDa
Background of B2M
Beta-2-microglobulin is a serum protein found in association with the major histocompatibility complex (MHC) class I heavy chain on the surface of nearly all nucleated cells.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00456-6 is guaranteed for Flow Cytometry, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:1000
IHC-P: 1:10-1:50
FC: 1:10-1:50
Validation Images & Assay Conditions
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B2M Antibody (C-term) western blot analysis in Jurkat cell line lysates (35ug/lane). This demonstrates the B2M antibody detected the B2M protein (arrow).
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Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with B2M antibody (C-term) (Cat. #A00456-1), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
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B2M Antibody (C-term) (Cat. #A00456-1) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Specific Publications For Anti-B2M Antibody (C-term) (A00456-6)
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