Anti-B9 domain-containing protein 1 B9D1 Antibody

Product Name

Anti-B9 domain-containing protein 1 B9D1 Antibody

View all B9D1 Antibodies

SKU/Catalog Number

A10722

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-B9 domain-containing protein 1 B9D1 Antibody (Catalog # A10722). Tested in ELISA, WB, ICC, IF applications. This antibody reacts with Human.

Storage & Handling

B9D1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Cite This Product

Anti-B9 domain-containing protein 1 B9D1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A10722)

Host

Rabbit

Contents

B9D1 Antibody is supplied in PBS containing 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Immunogen

B9D1 antibody was raised against an 18 amino acid synthetic peptide near the carboxy terminus of human B9D1. The immunogen is located within amino acids 130 - 180 of B9D1.

Cross-reactivity

At least two isoforms of B9D1 are known to exist; this antibody will only recognize the longest isoform. B9D1 antibody is predicted to not cross-react with other DNAJC family members.

Reactive Species

A10722 is reactive to B9D1 in Human

Observed Molecular Weight

68 kDa

Calculated molecular weight

22.8 kDa

Background of B9D1

Meckel syndrome (MKS) is an embryonic lethal, autosomal recessive disorder characterized by polycystic kidney disease, central nervous system defects, polydactyly and liver fibrosis. B9D1 is a B9 domain-containing protein, one of several that are involved in ciliogenesis. Alterations in expression of this gene have been found in a family with Meckel syndrome. B9D1, and its related protein B9D2, form a complex with MKS1, disruption of which causes MKS. B9D1 is thought to be required for normal hedgehog signaling, ciliogenesis, and ciliary protein localization.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A10722 is guaranteed for ELISA, IF, ICC, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

B9D1 antibody can be used for detection of B9D1 by Western blot at 1 μg/mL. Antibody can also be used for immunocytochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.

Validation Images & Assay Conditions

See full target information B9D1

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