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- Table of Contents
BAD antibody (M03520) performs well in Flow Cytometry, IP, IF, IHC, ICC, WB and shows reactivity with Human, Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 12 Publications
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Immunofluorescent analysis of Hela cells, using Bad Antibody .
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Anti-Bad Rabbit Monoclonal Antibody
BAD antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse, Rat | Rabbit | Flow Cytometry, IP, IF, IHC, ICC, WB |
Tested Applications
BAD antibody was positively detected by WB in:
human Hela whole cell, human MCF-7 whole cell
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
BAD antibody was positively detected by IHC in:
human breast cancer tissue, human placenta tissue, human placenta tissue, mouse small intestine tissue
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
BAD antibody was positively detected by ICC/IF in:
Hela cell
Recommend Dilution
Application
Dilution
Species
WB 1:500-2000
IHC 1:50-200
ICC/IF 1:50-200
IP 1:30
FC 1:50
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Reactive Data
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WB
IHC
IF
IP
FC
Human
Tested
Tested
Recommend
Recommend
Recommend
Mouse
Tested
Recommend
Recommend
Recommend
Rat
Recommend
Recommend
Product Information
Product Name
Anti-Bad Rabbit Monoclonal Antibody
View all BAD Antibodies
View all BAD Antibodies
Form
Liquid
Uniprot
Gene ID
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
CHC-2
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Bad
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
23 kDa
Calculated Molecular Weight
18.4 kDa
Cite This Product
Anti-Bad Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M03520)
Protocols
Western Blot protocol for BAD antibody
Immunohistochemistry (IHC) protocol for BAD antibody
Immunocytochemistry/Immunofluorescence (ICC/IF) protocol for BAD antibody
Publications
Application:
Species:
Rhodiola rosea L. Attenuates Cigarette Smoke and Lipopolysaccharide-Induced COPD in Rats via Inflammation Inhibition and Antioxidant and Antifibrosis Pathways
Published: 2021-03-02
Journal: Evidence-based Complementary and Alternative Medicine
Application: WB
Impact Factor: 2.63
Species: Rat
PMID: 33747104
Piperine Inhibits Cell Proliferation and Induces Apoptosis of Human Gastric Cancer Cells by Downregulating Phosphatidylinositol 3-Kinase (PI3K)/Akt Pathway
Published: 2020-12-31
Journal: MEDICAL SCIENCE MONITOR
Application: WB
Impact Factor: 1.92
Species: Human
PMID: 33382670
Combinatorial usage of fungal polysaccharides from Cordyceps sinensis and Ganoderma atrum ameliorate drug-induced liver injury in mice
Published: 2018-05-16
Journal: FOOD AND CHEMICAL TOXICOLOGY
Application: WB
Impact Factor: 3.98
Species: Mouse
PMID: 29753871
Comparative analysis of two ferritin subunits from blunt snout bream (Megalobrama amblycephala): Characterization, expression, iron depriving and bacteriostatic activity
Published: 2017-05-20
Journal: FISH & SHELLFISH IMMUNOLOGY
Impact Factor: 3.15
PMID: 28535971
The natural secolignan peperomin E induces apoptosis of human gastric carcinoma cells via the mitochondrial and PI3K/Akt signaling pathways in vitro and in vivo
Published: 2016-05-11
Journal: PHYTOMEDICINE
Application: WB
Impact Factor: 2.94
Species: Human
PMID: 27288917
The Viral Oncoprotein HBx of Hepatitis B virus Promotes the Growth of Hepatocellular Carcinoma through Cooperating with the Cellular Oncoprotein RMP
Published: 2014-11-18
Journal: International Journal of Biological Sciences
Application: WB
Impact Factor: 4.37
Species: Human
PMID: 25516716
Toxicological effects of cigarette smoke on Ana-1 macrophages in vitro
Published: 2013-03-19
Journal: EXPERIMENTAL AND TOXICOLOGIC PATHOLOGY
Application: WB
Impact Factor: 2.62
Species: Mouse
PMID: 23517752
Preventative Effects of 4,4'-Diphenylmethane-bis(methyl) Carbamate Isolated from Cortex Mori on Human Umbilical Vein Endothelial Cell Dysfunction Induced by Advanced Glycation End Products
Published: 2011-07-28
Journal: PHYTOTHERAPY RESEARCH
Application: ICC
Impact Factor: 1.88
Species: Human
PMID: 21796706
Phytoestrogen calycosin-7-O-β-D-glucopyranoside ameliorates advanced glycation end products-induced HUVEC damage
Published: 2011-06-06
Journal: JOURNAL OF CELLULAR BIOCHEMISTRY
Application: ICC
Impact Factor: 3.12
Species: Human
PMID: 21647942
Oleanolic acid from Prunella Vulgaris L. induces SPC-A-1 cell line apoptosis via regulation of Bax, Bad and Bcl-2 expression.
Published: 2011-01-01
Journal: Asian Pacific Journal of Cancer Prevention
Application: ICC
Impact Factor: 1.24
Species: Human
PMID: 21545203
Mechanism of Intracellular Signal Transduction during Injury of Renal Tubular Cells Induced by Postasphyxial Serum in Neonates with Asphyxia
Published: 2009-02-10
Journal: Neonatology
Application: ICC
Impact Factor: 1.92
Species: Human
PMID: 19204408
ACTX-8, a cytotoxic l-amino acid oxidase isolated from Agkistrodon acutus snake venom, induces apoptosis in Hela cervical cancer cells
Published: 2007-01-12
Journal: LIFE SCIENCES
Application: WB
Impact Factor: 2.39
Species: Human
PMID: 17275856
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Product Promise
We promise Anti-Bad Rabbit Monoclonal Antibody performs excellently in relevant applications for Flow Cytometry, IP, IF, IHC, ICC, WB. If it doesn't perform as promised, we will refund the product. Learn more
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M03520
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
Boster Guarantee
Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 3, 2026