Anti-BAF250B ARID1B Antibody

ARID1B antibody

Boster Bio Anti-BAF250B ARID1B Antibody (Catalog# A30528). Tested in WB, IHC-P, IF, ICC, ELISA applications. This antibody reacts with Human, Rat.

Product Info Summary

SKU: A30528
Size: 100ul
Reactive Species: Human, Rat
Host: Rabbit
Application: ELISA, IF, IHC-P, ICC, WB

Product Name

Anti-BAF250B ARID1B Antibody

View all ARID1B Antibodies

SKU/Catalog Number

A30528

Size

100ul

Form

Liquid

Description

Boster Bio Anti-BAF250B ARID1B Antibody (Catalog# A30528). Tested in WB, IHC-P, IF, ICC, ELISA applications. This antibody reacts with Human, Rat.

Storage & Handling

Store at -20°C for one year. For short-term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-BAF250B ARID1B Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A30528)

Host

Rabbit

Contents

PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A peptide derived from human BAF250B. Immunogen sequence location: 1371-1420

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A30528 is reactive to ARID1B in Human, Rat

Applications

A30528 is guaranteed for ELISA, IF, IHC-P, ICC, WB Boster Guarantee

Observed Molecular Weight

72 kDa

Calculated molecular weight

236.123kDa

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western Blot, 1:500 - 1:2000
Immunohistochemistry, 1:100 - 1:300
Immunofluorescence, 1:200 - 1:1000
ELISA, 1:10000
Not yet tested in other applications.

Validation Images & Assay Conditions

Gene/Protein Information For ARID1B (Source: Uniprot.org, NCBI)

Gene Name

ARID1B

Full Name

AT-rich interactive domain-containing protein 1B

Weight

236.123kDa

Alternative Names

6A3-5; ARID domain-containing protein 1B; AT rich interactive domain 1B (SWI1-like); BAF250B; BRG1-associated factor 250b; BRG1-binding protein ELD/OSA1; BRG1-binding protein hELD/OSA1; BRIGHT; DAN15P250R; ELD (eyelid)/OSA protein; ELD/OSA1; hOsa2; JPUT1; KDM5B; KIAA1235AT-rich interactive domain-containing protein 1B; Osa homolog 2; OSA2; p250R; PLU1; RBBP2H1A ARID1B 6A3-5, BAF250B, BRIGHT, CSS1, DAN15, ELD/OSA1, MRD12, OSA2, P250R AT-rich interaction domain 1B AT-rich interactive domain-containing protein 1B|ARID domain-containing protein 1B|AT rich interactive domain 1B (SWI1-like)|BRG1-associated factor 250b|BRG1-binding protein ELD/OSA1|ELD (eyelid)/OSA protein

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on ARID1B, check out the ARID1B Infographic

ARID1B infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for ARID1B: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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3 Customer Q&As for Anti-BAF250B ARID1B Antibody

Question

I was wanting to use your anti-BAF250B antibody for WB for human cervix carcinoma erythroleukemia on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human cervix carcinoma erythroleukemia identification?

Verified Customer

Verified customer

Asked: 2019-12-04

Answer

You can see on the product datasheet, A30528 anti-BAF250B antibody has been tested for IF, WB on human, rat tissues. We have an innovator award program that if you test this antibody and show it works in human cervix carcinoma erythroleukemia in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2019-12-04

Question

We are currently using anti-BAF250B antibody A30528 for human tissue, and we are well pleased with the IF results. The species of reactivity given in the datasheet says human, rat. Is it likely that the antibody can work on pig tissues as well?

E. Thomas

Verified customer

Asked: 2015-11-20

Answer

The anti-BAF250B antibody (A30528) has not been validated for cross reactivity specifically with pig tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2015-11-20

Question

Can you help my question with product A30528, anti-BAF250B antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

G. Moore

Verified customer

Asked: 2014-09-30

Answer

We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A30528 anti-BAF250B antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2014-09-30

Order DetailsPrice
A30528

100 μL

$335

Free Secondary Antibody

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Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
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