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Product Info Summary
| SKU: | A30528 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-BAF250B ARID1B Antibody
SKU/Catalog Number
A30528
Size
100ul
Form
Liquid
Description
Boster Bio Anti-BAF250B ARID1B Antibody (Catalog# A30528). Tested in WB, IHC, ICC, IF, ELISA applications. This antibody reacts with Human, Rat.
Storage & Handling
Store at -20°C for one year. For short-term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-BAF250B ARID1B Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A30528)
Host
Rabbit
Contents
PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A peptide derived from human BAF250B. Immunogen sequence location: 1371-1420
Reactive Species
A30528 is reactive to ARID1B in Human, Rat
Observed Molecular Weight
72 kDa
Calculated molecular weight
236.1 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A30528 is guaranteed for ELISA, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500 - 1:2000.
IHC 1:100 - 1:300.
ICC/IF 1:200 - 1:1000.
ELISA: 1:10000.
Not yet tested in other applications.
Validation Images & Assay Conditions
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Immunohistochemical analysis of paraffin-embedded Human breast cancer. Antibody was diluted at 1:100(4° overnight). High-pressure and temperature Tris-EDTA,pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.
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Western Blot analysis of 3T3 cells using BAF250b Polyclonal Antibody diluted at 1:1000
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Immunofluorescence analysis of HUVEC cells, using BAF250B Antibody. The picture on the right is blocked with the synthesized peptide.
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Western blot analysis of lysates from LOVO cells, using BAF250B Antibody. The lane on the right is blocked with the synthesized peptide.
Specific Publications For Anti-BAF250B ARID1B Antibody (A30528)
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Customer Q&As
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3 Customer Q&As for Anti-BAF250B ARID1B Antibody
Question
I was wanting to use your anti-BAF250B antibody for WB for human cervix carcinoma erythroleukemia on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human cervix carcinoma erythroleukemia identification?
Verified Customer
Verified customer
Asked: 2019-12-04
Answer
You can see on the product datasheet, A30528 anti-BAF250B antibody has been tested for IF, WB on human, rat tissues. We have an innovator award program that if you test this antibody and show it works in human cervix carcinoma erythroleukemia in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-12-04
Question
We are currently using anti-BAF250B antibody A30528 for human tissue, and we are well pleased with the IF results. The species of reactivity given in the datasheet says human, rat. Is it likely that the antibody can work on pig tissues as well?
E. Thomas
Verified customer
Asked: 2015-11-20
Answer
The anti-BAF250B antibody (A30528) has not been validated for cross reactivity specifically with pig tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2015-11-20
Question
Can you help my question with product A30528, anti-BAF250B antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
G. Moore
Verified customer
Asked: 2014-09-30
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A30528 anti-BAF250B antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2014-09-30
