Anti-BAL/SLC27A5 Antibody Picoband™

Product Name

Anti-BAL/SLC27A5 Antibody Picoband™

View all FATP5/SLC27A5 Antibodies

SKU/Catalog Number

A09287-2

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-BAL/SLC27A5 Antibody Picoband™ catalog # A09287-2. Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human, Rat.

Storage & Handling

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Cite This Product

Anti-BAL/SLC27A5 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A09287-2)

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived human BAL/SLC27A5 recombinant protein (Position: I204-L690).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A09287-2 is reactive to SLC27A5 in Human, Rat

Applications

A09287-2 is guaranteed for ELISA, Flow Cytometry, WB Boster Guarantee

Observed Molecular Weight

75 kDa

Calculated molecular weight

75.385kDa

Background of FATP5/SLC27A5

Bile acyl-CoA synthetase is an enzyme that in humans is encoded by the SLC27A5 gene. The protein encoded by this gene is an isozyme of very long-chain acyl-CoA synthetase (VLCS). It is capable of activating very long-chain fatty-acids containing 24- and 26-carbons. It is expressed in liver and associated with endoplasmic reticulum but not with peroxisomes. Its primary role is in fatty acid elongation or complex lipid synthesis rather than in degradation. This gene has a mouse ortholog.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.25-0.5 µg/ml, Human, Rat
Flow Cytometry, 1-3 µg/1x10⁶ cells, Human
Direct ELISA, 0.1-0.5 µg/ml, Human

Validation Images & Assay Conditions

Click image to see more details

Figure 1. Western blot analysis of BAL/SLC27A5 using anti-BAL/SLC27A5 antibody (A09287-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample unde
r reducing conditions.
Lane 1: human hepatocellular carcinoma paracancerous tissue (HCCP) lysates,
Lane 2: rat liver tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-BAL/SLC27A5 antigen affinity purified polyclonal antibody (Catalog # A09287-2) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for BAL/SLC27A5 at approximately 75 kDa. The expected band size for BAL/SLC27A5 is at 75 kDa.

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Figure 2. Flow Cytometry analysis of Jurkat cells using anti-BAL/SLC27A5 antibody (A09287-2).
Overlay histogram showing Jurkat cells stained with A09287-2 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-BAL/SLC27A5 Antibody (A09287-2, 1 μg/1x10⁶ cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x10⁶ cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x10⁶) used under the same conditions. Unlabelled sample (Red line) was also used as a control.

Gene/Protein Information For SLC27A5 (Source: Uniprot.org, NCBI)

Alternative Names

ACSB; ACSBBACS; ACSVL6; ACSVL6BAL; BA-CoA ligase; Bile acid-CoA ligase; bile acyl-CoA synthetase; Cholate--CoA ligase; EC 6.2.1.7; FACVL3; FACVL3FATP-5; FATP5; FATP5Solute carrier family 27 member 5; Fatty acid transport protein 5; Fatty-acid-coenzyme A ligase, very long-chain 3; FLJ22987; SLC27A5; solute carrier family 27 (fatty acid transporter), member 5; VLACSR; VLACSRVery long-chain acyl-CoA synthetase homolog 2; VLCSH2; VLCSH2Very long-chain acyl-CoA synthetase-related protein; VLCS-H2VLACS-related SLC27A5 ACSB, ACSVL6, BACS, BAL, FACVL3, FATP-5, FATP5, VLACSR, VLCS-H2, VLCSH2 solute carrier family 27 member 5 bile acyl-CoA synthetase|BA-CoA ligase|VLACS-related|bile acid-CoA ligase|cholate--CoA ligase|fatty acid transport protein 5|fatty-acid-Coenzyme A ligase, very long-chain 3|solute carrier family 27 (fatty acid transporter), member 5|very long-chain acyl-CoA synthetase homolog 2|very long-chain acyl-CoA synthetase-related protein

For more info on SLC27A5, check out the SLC27A5 Infographic

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In this infographic, you will see the following information for SLC27A5: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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