Anti-BAPX1 NKX3-2 Antibody

Product Name

Anti-BAPX1 NKX3-2 Antibody

View all NKX3-2 Antibodies

SKU/Catalog Number

A07429

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-BAPX1 NKX3-2 Antibody (Catalog # A07429). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Antibody can be stored at 4°C up to one year. Antibodies should not be exposed to prolonged high temperatures.

Cite This Product

Anti-BAPX1 NKX3-2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A07429)

Host

Rabbit

Contents

BAPX1 Antibody is supplied in PBS containing 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Immunogen

BAPX1 antibody was raised against a 19 amino acid synthetic peptide near the center of human BAPX1. The immunogen is located within amino acids 180 - 230 of BAPX1.

Cross-reactivity

BAPX1 antibody is predicted not to cross-react with other NKX homeobox proteins.

Reactive Species

A07429 is reactive to NKX3-2 in Human, Mouse, Rat

Observed Molecular Weight

68 kDa

Calculated molecular weight

34.8 kDa

Background of NKX3-2

BAPX1 is the mammalian homolog of the Drosophila bagpipe homeobox gene and is expressed in the splanchnic mesoderm and embryonic skeleton. It is one of the earliest developmental markers for the sclerotome portion of the somite and the gut mesentery. BAPX1 is required for normal skeletal development; homozygous inactivating mutations in the BAPX1 gene result in spodylo-megaepiphyseal-metaphyseal dysplasia (SMMD). It has also been suggested to play a role in the proper development of the mammalian gut and is required for distal stomach development as part of a BARX1-dependent pathway.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A07429 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

BAPX1 antibody can be used for detection of BAPX1 by Western blot at 1 and 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.

Validation Images & Assay Conditions

See full target information NKX3-2

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