Anti-BATF3/Snft Antibody

Product Name

Anti-BATF3/Snft Antibody

View all BATF3 Antibodies

SKU/Catalog Number

A01957

Size

100μl

Form

Liquid

Description

Boster Bio Anti-BATF3/Snft Antibody catalog # A01957. Tested in ELISA, IHC-P, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-BATF3/Snft Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01957)

Host

Rabbit

Contents

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Immunogen

Synthesized peptide derived from the Internal region of human BATF3.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross reactivity with other proteins.

Reactive Species

A01957 is reactive to BATF3 in Human, Mouse, Rat

Applications

A01957 is guaranteed for ELISA, IHC-P, WB Boster Guarantee

Observed Molecular Weight

39 kDa

Calculated molecular weight

14.468kDa

Background of BATF3

CaM Kinase IV (also known as CAM kinase-GR and CaMK IV) is a calcium/ calmodulin-dependent protein kinase belonging to a proposed calcium-triggered signaling cascade. This kinase may be involved in the transcriptional regulation of microtubule dynamics. In vitro, CaMK IV phosphorylates CREB1, CREBBP, PRM2, MEF2A, MEF2D and STMN1/OP18. CaMK IV may also be involved in spermatogenesis and may play a role in the consolidation/ retention of hippocampus-dependent long-term memory. CaMK IV must be phosphorylated to be maximally active and is phosphorylated by CAMKK1 or CAMKK2. In addition autophosphorylation of the N-terminus is required for full activation. Autophosphorylation of Ser-336 allows the kinase to switch to a Ca(2+)/calmodulin-independent state. Most likely the kinase is inactivated by the serine/ threonine protein phosphatase 2A. CaMK IV is a monomer that is located within the cytoplasm and nucleus and substantial localization occurs in certain neuronal nuclei. In spermatids CaMK IV is associated with chromatin and the nuclear matrix. CaMK IV is also specifically expressed in epithelial ovarian cancer tissue.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Restore with deionized water (or equivalent) for reconstitution volume of 100 µL

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB, 1:500-1:2000
IHC-P, 1:100-1:300

Validation Images & Assay Conditions

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Immunohistochemistry (IHC) analysis of paraffin-embedded Human Breast Cancer, antibody was diluted at 1:200.

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Western blot analysis of mouse-lung mouse-spleen lysis using BATF3 antibody. Antibody was diluted at 1:2000. Secondary antibody was diluted at 1:20000

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Immunohistochemistry (IHC) analysis of paraffin-embedded Human Breast Cancer, antibody was diluted at 1:200.

Gene/Protein Information For BATF3 (Source: Uniprot.org, NCBI)

Alternative Names

basic leucine zipper transcription factor, ATF-like 3; basic leucine zipper transcriptional factor ATF-like 3; BATF3; B-ATF-3; FLJ36352; FLJ37535,21 kDa small nuclear factor isolated from T-cells; JDP1; Jun dimerization protein 1; Jun dimerization protein p21SNFT; JUNDM1; SNFT; SNFT21-kD small nuclear factor isolated from T cells BATF3 JDP1, JUNDM1, SNFT basic leucine zipper ATF-like transcription factor 3 basic leucine zipper transcriptional factor ATF-like 3|21 kDa small nuclear factor isolated from T-cells|21-kD small nuclear factor isolated from T cells|B-ATF-3|Jun dimerization protein 1|Jun dimerization protein p21SNFT|basic leucine zipper transcription factor, ATF-like 3

For more info on BATF3, check out the BATF3 Infographic

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In this infographic, you will see the following information for BATF3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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