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Product Info Summary
| SKU: | M00183 |
|---|---|
| Size: | 100ug/vial |
| Reactive Species: | Human, Monkey |
| Host: | Mouse |
| Application: | Flow Cytometry, IHC |
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Product info
Product Name
Anti-Bax (Apoptosis Marker) Monoclonal Antibody
SKU/Catalog Number
M00183
Size
100ug/vial
Form
Liquid
Description
Boster Bio Anti-Bax (Apoptosis Marker) Monoclonal Antibody (Catalog # M00183). Tested in Flow Cytometry, IHC applications. This antibody reacts with Human, Monkey.
Storage & Handling
Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Cite This Product
Anti-Bax (Apoptosis Marker) Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00183)
Host
Mouse
Contents
Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Clonality
Monoclonal
Clone Number
Clone: BAX/962
Isotype
IgG1, kappa
Immunogen
Recombinant full-length human BAX protein.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00183 is reactive to BAX in Human, Monkey
Applications
M00183 is guaranteed for Flow Cytometry, IHC Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
21.184kDa
Background of BAX
Recognizes a protein of 21kDa, identified as the Bax protein. This monoclonal antibody is highly specific to Bax and shows no cross-reaction with Bcl-2 or Bcl-X protein. Bcl-2 blocks cell death following a variety of stimuli. Bax has extensive amino acid homology with Bcl-2 and it homodimerizes and forms heterodimers with Bcl-2. Overexpression of Bax accelerates apoptotic death induced by cytokine deprivation in an IL-3 dependent cell line, and Bax also counters the death repressor activity of Bcl-2.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
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Assay dilution & Images
Reconsitution
Restore with deionized water (or equivalent) for reconstitution volume of 100 µL
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Flow Cytometry (1-2ug/million cells)
Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT) (Staining of formalin-fixed tissues requires heating tissue sections in 1mM EDTA buffer, pH 8.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.
Validation Images & Assay Conditions
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Formalin-fixed, paraffin-embedded human Melanoma stained with Anti-BAX Mouse Monoclonal Antibody
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Formalin-fixed, paraffin-embedded Hodgkin's Lymphoma stained with Anti-BAX Mouse Monoclonal Antibody (BAX/962).
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Flow Cytometric analysis of Jurkat cells using Anti-Bax Mouse Monoclonal Antibody followed by Goat anti-mouse IgG-CF488 (Blue); Isotype Control (Red).
Protein Target Info & Infographic
Gene/Protein Information For BAX (Source: Uniprot.org, NCBI)
Gene Name
BAX
Full Name
Apoptosis regulator BAX
Weight
21.184kDa
Superfamily
Bcl-2 family
Alternative Names
apoptosis regulator BAX; Bax; BCL2-associated X protein; Bcl2-L-4; BCL2L4bcl2-L-4; Bcl-2-like protein 4 BAX BCL2L4 BCL2 associated X, apoptosis regulator apoptosis regulator BAX|BCL2 associated X protein|BCL2-associated X protein omega|Baxdelta2G9|Baxdelta2G9omega|Baxdelta2omega|bcl-2-like protein 4|bcl2-L-4
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on BAX, check out the BAX Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for BAX: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Bax (Apoptosis Marker) Monoclonal Antibody (M00183)
Hello CJ!
M00183 has been cited in 18 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Wu J,Qu JQ,Zhou YJ,Zhou YJ,Li YY,Huang NQ,Deng CM,Luo Y.Icariin improves cognitive deficits by reducing the deposition of β-amyloid peptide and inhibition of neurons apoptosis in SAMP8 mice.Neuroreport.2020 Jun 7;31(9):663-671.doi:10.1097/WNR.000000000000
Species: Mouse
M00183 usage in article: APP:IHC, SAMPLE:HIPPOCAMPUS TISSUE, DILUTION:1:200
Chai BY,Gong FK,Chen ZH,Li ZX,Zhang B.System Prediction and Validation of TCM for Chronic Myeloid Leukemia Treatment from the Perspective of Low-Toxicity Chemotherapy: A Stilbene α-Viniferin Has a Proapoptotic Effect on K562 Cells via the Mitochondrial Pa
Species: Human
M00183 usage in article: APP:WB, SAMPLE:K562 CELL, DILUTION:1:400
Wang P,Wang C,Liu C. Antitumor effects of dioscin in A431 cells via adjusting ATM/p53-mediated cell apoptosis, DNA damage and migration. Oncol Lett. 2021 Jan;21(1):59.doi:10.3892/ol.2020.12321.Epub 2020 Nov 19.PMID:33281970;PMCID:PMC7709553.
Species: Human
M00183 usage in article: APP:WB, SAMPLE:A431 CELLS, DILUTION:NA
Newcastle disease virus V protein inhibits apoptosis in DF-1 cells by downregulating TXNL1
The Effects of Fasudil at Different Doses on Acute Myocardial Infarction in Rats
A preliminary study of the effect of ECRG4 overexpression on the proliferation and apoptosis of human laryngeal cancer cells and the underlying mechanisms
In vitro and in vivo antitumor effects of acetylshikonin isolated from Arnebia euchroma (Royle) Johnst (Ruanzicao) cell suspension cultures
Croton Tiglium Extract Induces Apoptosis via Bax/Bcl-2 Pathways in Human Lung Cancer A549 Cells
Knockdown of High Mobility Group-Box 3 (HMGB3) Expression Inhibits Proliferation, Reduces Migration, and Affects Chemosensitivity in Gastric Cancer Cells
Mesenchymal stem cells promote colorectal cancer progression through AMPK/mTOR-mediated NF-?B activation
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Customer Reviews
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Customer Q&As
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Find answers in Q&As, reviews.
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16 Customer Q&As for Anti-Bax (Apoptosis Marker) Monoclonal Antibody
Question
Is a blocking peptide available for product anti-Bax (Apoptosis Marker) Monoclonal antibody (M00183)?
Verified Customer
Verified customer
Asked: 2020-02-10
Answer
We do provide the blocking peptide for product anti-Bax (Apoptosis Marker) Monoclonal antibody (M00183). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2020-02-10
Question
I was wanting to use to test anti-Bax (Apoptosis Marker) Monoclonal antibody M00183 on monkey ovarian carcinoma for research purposes, then I may be interested in using anti-Bax (Apoptosis Marker) Monoclonal antibody M00183 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2020-01-21
Answer
The products we sell, including anti-Bax (Apoptosis Marker) Monoclonal antibody M00183, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2020-01-21
Question
Is this M00183 anti-Bax (Apoptosis Marker) Monoclonal antibody reactive to the isotypes of BAX?
Verified Customer
Verified customer
Asked: 2019-12-23
Answer
The immunogen of M00183 anti-Bax (Apoptosis Marker) Monoclonal antibody is Recombinant full-length human BAX protein. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-12-23
Question
My boss were satisfied with the WB result of your anti-Bax (Apoptosis Marker) Monoclonal antibody. However we have observed positive staining in ovarian carcinoma isoform delta: cytoplasm. using this antibody. Is that expected? Could you tell me where is BAX supposed to be expressed?
Verified Customer
Verified customer
Asked: 2019-11-06
Answer
According to literature, ovarian carcinoma does express BAX. Generally BAX expresses in isoform alpha: mitochondrion outer membrane, isoform beta: cytoplasm., isoform gamma: cytoplasm., isoform delta: cytoplasm. Regarding which tissues have BAX expression, here are a few articles citing expression in various tissues:
B-cell, Pubmed ID: 8358790
Brain, Pubmed ID: 9920818
Ovarian carcinoma, Pubmed ID: 14702039
Skin, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2019-11-06
Question
We have observed staining in monkey skin. Any tips? Is anti-Bax (Apoptosis Marker) Monoclonal antibody supposed to stain skin positively?
Verified Customer
Verified customer
Asked: 2019-09-26
Answer
From what I have seen in literature skin does express BAX. From what I have seen in Uniprot.org, BAX is expressed in mucosa of transverse colon, b-cell, brain, ovarian carcinoma, skin, among other tissues. Regarding which tissues have BAX expression, here are a few articles citing expression in various tissues:
B-cell, Pubmed ID: 8358790
Brain, Pubmed ID: 9920818
Ovarian carcinoma, Pubmed ID: 14702039
Skin, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2019-09-26
Question
Please see the WB image, lot number and protocol we used for ovarian carcinoma using anti-Bax (Apoptosis Marker) Monoclonal antibody M00183. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-07-04
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-07-04
Question
I was wanting to use your anti-Bax (Apoptosis Marker) Monoclonal antibody for IHC for monkey ovarian carcinoma on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for monkey ovarian carcinoma identification?
Verified Customer
Verified customer
Asked: 2019-03-06
Answer
It shows on the product datasheet, M00183 anti-Bax (Apoptosis Marker) Monoclonal antibody has been validated for IF, IHC on human, monkey tissues. We have an innovator award program that if you test this antibody and show it works in monkey ovarian carcinoma in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-03-06
Question
Will M00183 anti-Bax (Apoptosis Marker) Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
C. Miller
Verified customer
Asked: 2019-01-16
Answer
It shows on the product datasheet, M00183 anti-Bax (Apoptosis Marker) Monoclonal antibody as been tested on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-01-16
Question
I see that the anti-Bax (Apoptosis Marker) Monoclonal antibody M00183 works with IHC, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2018-11-12
Answer
You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2018-11-12
Question
We are currently using anti-Bax (Apoptosis Marker) Monoclonal antibody M00183 for monkey tissue, and we are happy with the IHC results. The species of reactivity given in the datasheet says human, monkey. Is it true that the antibody can work on monkey tissues as well?
Verified Customer
Verified customer
Asked: 2018-08-02
Answer
The anti-Bax (Apoptosis Marker) Monoclonal antibody (M00183) has not been validated for cross reactivity specifically with monkey tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-08-02
Question
Do you have a BSA free version of anti-Bax (Apoptosis Marker) Monoclonal antibody M00183 available?
Verified Customer
Verified customer
Asked: 2018-07-02
Answer
Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-Bax (Apoptosis Marker) Monoclonal antibody M00183 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2018-07-02
Question
Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for ovarian carcinoma using anti-Bax (Apoptosis Marker) Monoclonal antibody M00183. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2017-09-18
Answer
I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-09-18
Question
We bought anti-Bax (Apoptosis Marker) Monoclonal antibody for IHC on mucosa of transverse colon in the past. I am using human, and I plan to use the antibody for IF next. We want examining mucosa of transverse colon as well as skin in our next experiment. Could you please give me some suggestion on which antibody would work the best for IF?
L. Evans
Verified customer
Asked: 2016-10-28
Answer
I have checked the website and datasheets of our anti-Bax (Apoptosis Marker) Monoclonal antibody and it appears that M00183 has been tested on human in both IHC and IF. Thus M00183 should work for your application. Our Boster satisfaction guarantee will cover this product for IF in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for IF detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2016-10-28
Question
Would anti-Bax (Apoptosis Marker) Monoclonal antibody M00183 work for IHC with ovarian carcinoma?
L. Dhar
Verified customer
Asked: 2016-09-23
Answer
According to the expression profile of ovarian carcinoma, BAX is highly expressed in ovarian carcinoma. So, it is likely that anti-Bax (Apoptosis Marker) Monoclonal antibody M00183 will work for IHC with ovarian carcinoma.
Boster Scientific Support
Answered: 2016-09-23
Question
Can you help my question with product M00183, anti-Bax (Apoptosis Marker) Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
M. Bhatt
Verified customer
Asked: 2014-09-30
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M00183 anti-Bax (Apoptosis Marker) Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2014-09-30
Question
We want using your anti-Bax (Apoptosis Marker) Monoclonal antibody for kidney development studies. Has this antibody been tested with western blotting on human jurkat cells? We would like to see some validation images before ordering.
C. Krishna
Verified customer
Asked: 2013-02-13
Answer
I appreciate your inquiry. This M00183 anti-Bax (Apoptosis Marker) Monoclonal antibody is validated on human jurkat cells. It is guaranteed to work for IF, IHC in human, monkey. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2013-02-13
