Anti-Bcl-2-modifying factor Bmf Antibody

Product Name

Anti-Bcl-2-modifying factor Bmf Antibody

View all BMF Antibodies

SKU/Catalog Number

A02432-1

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-Bcl-2-modifying factor Bmf Antibody (Catalog # A02432-1). Tested in ELISA, WB, ICC applications. This antibody reacts with Human, Mouse.

Storage & Handling

Bmf antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Cite This Product

Anti-Bcl-2-modifying factor Bmf Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02432-1)

Host

Rabbit

Contents

Bmf Antibody is supplied in PBS containing 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Immunogen

Bmf antibody was raised with a synthetic peptide corresponding to 15 amino acids near the amino terminus of human Bmf. The immunogen is located within the first 50 amino acids of Bmf.

Reactive Species

A02432-1 is reactive to BMF in Human, Mouse

Observed Molecular Weight

68 kDa

Calculated molecular weight

20.5 kDa

Background of BMF

Apoptosis is related to many diseases and development. Members in the Bcl-2 family are critical regulators of apoptosis by either inhibiting or promoting cell death. Bcl-2 homology 3 (BH3) domain is a potent death domain. BH3-only proteins, including Bad, Bid, Bik, Hrk, Bim, Noxa, and PUMA, form a growing subclass of the Bcl-2 family. A novel BH3-only protein was recently identified in human and mouse and designated Bmf (for Bcl-2-modifing factor). The BH3 domain in Bmf is required both for binding to Bcl-2 proteins and for triggering apoptosis. In healthy cells, Bmf associates with the dynein light chain 2 (DLC2) component of the myosin V motors and is sequestered by the cell's actin cytoskeleton. Disruption of the actin cytoskeleton, either by depolymerization of actin filaments or by detachment of cells from the extracellular matrix, triggers release and activation of Bmf, initiating the downstream apoptotic program. Bmf is constitutively expressed in many tissues.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A02432-1 is guaranteed for ELISA, ICC, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Bmf antibody can be used for detection of Bmf by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunocytochemistry starting at 10 μg/mL. For immunofluorescence start at 5 μg/mL.
Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.

Validation Images & Assay Conditions

See full target information BMF

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