Anti-Bcl-rambo BCL2L13 Antibody

Product Name

Anti-Bcl-rambo BCL2L13 Antibody

View all BCL2L13 Antibodies

SKU/Catalog Number

A06151

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-Bcl-rambo BCL2L13 Antibody (Catalog # A06151). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Bcl-rambo antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Cite This Product

Anti-Bcl-rambo BCL2L13 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A06151)

Host

Rabbit

Contents

Bcl-rambo Antibody is supplied in PBS containing 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Immunogen

Bcl-rambo antibody was raised against a 15 amino acid synthetic peptide from near the center of human Bcl-rambo. The immunogen is located within amino acids 280 - 330 of Bcl-rambo.

Reactive Species

A06151 is reactive to BCL2L13 in Human, Mouse, Rat

Observed Molecular Weight

68 kDa

Calculated molecular weight

52.7 kDa

Background of BCL2L13

Apoptosis plays a major role in normal organism development, tissue homeostasis, and removal of damaged cells. Disruption of this process has been implicated in a variety of diseases such as cancer. Members of the Bcl-2 family are known to be critical regulators of this process. These proteins are characterized by the presence of several conserved motifs termed Bcl-2 homology (BH) domains. A novel, widely expressed member termed Bcl-rambo was recently identified. This protein is localized to mitochondria in mammalian cells and its overexpression induces apoptosis which could be blocked by co-expression of inhibitor of apoptosis proteins (IAPs) such as XIAP, cIAP1, and cIAP2. Bcl-rambo shows overall homology to the anti-apoptotic members containing BH motifs, but unlike Bcl-2, the C-terminal membrane anchor of Bcl-rambo is preceded by a unique 250 amino acid insertion. This region by itself can induce apoptosis more efficiently than the Bcl-2 homology regions, suggesting that Bcl-rambo may be important other pro-apoptotic pathways.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A06151 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Bcl-rambo antibody can be used for the detection of Bcl-rambo by Western blot at 2 and 4 μg/mL. Antibody can also be used for immunohistochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.

Validation Images & Assay Conditions

See full target information BCL2L13

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