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Product Info Summary
| SKU: | M01616 |
|---|---|
| Size: | 80 µl |
| Reactive Species: | Human |
| Host: | Mouse |
| Application: | Flow Cytometry, IHC-P, WB |
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Product info
Product Name
Anti-BCL10 Antibody
SKU/Catalog Number
M01616
Size
80 µl
Description
Boster Bio Anti-BCL10 Antibody (Catalog # M01616). Tested in IHC-P, Flow Cytometry, WB application(s). This antibody reacts with Human.
Storage & Handling
Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Cite This Product
Anti-BCL10 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01616)
Host
Mouse
Contents
Purified monoclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clonality
Monoclonal
Clone Number
1185CT13.2.1.2
Isotype
IgG1,κ
Immunogen
This BCL10 antibody is generated from a mouse immunized with a KLH conjugated synthetic peptide between 1-143 amino acids from the human region of human BCL10.
Reactive Species
M01616 is reactive to BCL10 in Human
Calculated molecular weight
26.3 kDa
Background of BCL10
Promotes apoptosis, pro-caspase-9 maturation and activation of NF-kappa-B via NIK and IKK. May be an adapter protein between upstream TNFR1-TRADD-RIP complex and the downstream NIK-IKK-IKAP complex. Is a substrate for MALT1.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M01616 is guaranteed for Flow Cytometry, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:2000
IHC-P: 1:25
FC: 1:25
Validation Images & Assay Conditions
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Western blot analysis of lysate from Daudi cell line using BCL10 Antibody. M01616 was diluted at 1:1000 at each lane. A goat anti-mouse IgG H&L (HRP) at 1:3000 dilution was used as the secondary antibody. Lysate at 35μg per lane.
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Immunohistochemical analysis of paraffin-embedded H. kidney section using BCL10 Antibody. M01616 was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-mouse IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
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Immunohistochemical analysis of paraffin-embedded M. kidney section using BCL10 Antibody. M01616 was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-mouse IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
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Flow cytometric analysis of Hela cells using BCL10 Antibody(green, Cat#M01616) compared to an isotype control of mouse IgG1(blue). M01616 was diluted at 1:25 dilution. An Alexa Fluor® 488 goat anti-mouse lgG at 1:400 dilution was used as the secondary antibody.
Specific Publications For Anti-BCL10 Antibody (M01616)
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