Anti-BCL6 Antibody (Center)

Product Name

Anti-BCL6 Antibody (Center)

View all Bcl-6 Antibodies

SKU/Catalog Number

M00142

Size

50 µl

Description

Boster Bio Anti-BCL6 Antibody (Center) (Catalog # M00142). Tested in WB, IHC-P application(s). This antibody reacts with Human, Mouse.

Storage & Handling

Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Cite This Product

Anti-BCL6 Antibody (Center) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00142)

Host

Rabbit

Contents

Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.

Clonality

Polyclonal

Clone Number

1723CT382.14.9

Isotype

Rabbit IgG

Immunogen

This BCL6 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 364-395 amino acids from the Central region of human BCL6.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

M00142 is reactive to BCL6 in Human, Mouse

Applications

M00142 is guaranteed for IHC-P, WB Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

78.846kDa

Background of Bcl-6

Transcriptional repressor mainly required for germinal center (GC) formation and antibody affinity maturation which has different mechanisms of action specific to the lineage and biological functions. Forms complexes with different corepressors and histone deacetylases to repress the transcriptional expression of different subsets of target genes. Represses its target genes by binding directly to the DNA sequence 5'-TTCCTAGAA-3' (BCL6- binding site) or indirectly by repressing the transcriptional activity of transcription factors. In GC B-cells, represses genes that function in differentiation, inflammation, apoptosis and cell cycle control, also autoregulates its transcriptional expression and up-regulates, indirectly, the expression of some genes important for GC reactions, such as AICDA, through the repression of microRNAs expression, like miR155. An important function is to allow GC B-cells to proliferate very rapidly in response to T-cell dependent antigens and tolerate the physiological DNA breaks required for immunglobulin class switch recombination and somatic hypermutation without inducing a p53/TP53-dependent apoptotic response. In follicular helper CD4(+) T-cells (T(FH) cells), promotes the expression of T(FH)-related genes but inhibits the differentiation of T(H)1, T(H)2 and T(H)17 cells. Also required for the establishment and maintenance of immunological memory for both T- and B-cells. Suppresses macrophage proliferation through competition with STAT5 for STAT-binding motifs binding on certain target genes, such as CCL2 and CCND2. In response to genotoxic stress, controls cell cycle arrest in GC B-cells in both p53/TP53- dependedent and -independent manners. Besides, also controls neurogenesis through the alteration of the composition of NOTCH- dependent transcriptional complexes at selective NOTCH targets, such as HES5, including the recruitment of the deacetylase SIRT1 and resulting in an epigenetic silencing leading to neuronal differentiation.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB: 1:1000
IHC-P: 1:25

Validation Images & Assay Conditions

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All lanes : Anti-BCL6 Antibody (Center) at 1:1000 dilution
Lane 1: mouse spleen lysate
Lane 2: Ramos whole cell lysate
Lane 3: Raji whole cell lysate
Lane 4: Jurkat whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 79 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.

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M00142 staining BCL6 in Human tonsil tissue sections by Immunohistochemistry (IHC-P -paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.

Gene/Protein Information For BCL6 (Source: Uniprot.org, NCBI)

Alternative Names

B-cell CLL/lymphoma 6; B-cell lymphoma 6 protein transcript; Bcl-5; BCL5B-cell lymphoma 5 protein; Bcl6; Bcl-6; BCL6A; cys-his2 zinc finger transcription factor; LAZ3; LAZ3ZBTB27B-cell lymphoma 6 protein; lymphoma-associated zinc finger gene on chromosome 3; Protein LAZ-3; ZBTB27 ; Zinc finger and BTB domain-containing protein 27; Zinc finger protein 51ZNF51; zinc finger transcription factor BCL6S; ZNF51 BCL6 BCL5A, LAZ3, ZBTB27, ZNF51, BCL6 BCL6 transcription repressor B-cell lymphoma 6 protein|B cell CLL/lymphoma 6|B-cell lymphoma 5 protein|B-cell lymphoma 6 protein transcript|BCL-5|BCL-6|cys-his2 zinc finger transcription factor|lymphoma-associated zinc finger gene on chromosome 3|protein LAZ-3|zinc finger and BTB domain-containing protein 27|zinc finger protein 51|zinc finger transcription factor BCL6S

For more info on BCL6, check out the BCL6 Infographic

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In this infographic, you will see the following information for BCL6: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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